Table 1.
Primers for quantitative PCR.
| Gene name | Forward/Reverse primers (5′–3′) | Amplicon (bp) | Primer efficiency1 (%) | Accession no. |
|---|---|---|---|---|
| Mucin 2 (Muc2) | CTGATTGTCACTCACGCCTTAATC/GCCGGCCACCTGCAT | 147 | 95.9 ± 4.5 | JX284122.1 |
| Peptide transporter 1 (PepT1) | CCCCTGAGGAGGATCACTGTT/CAAAAGAGCAGCAGCAACGA | 66 | 88.1 ± 4.7 | KF366603.1 |
| Ribosomal protein large subunit P1 (RPLP1) | TCTCCACGACGACGAAGTCA/CCGCCGCCTTGATGAG | 55 | 92.6 ± 5.6 | NM_205322.1 |
| Ribosomal protein large subunit 4 (RPL4) | TCAAGGCGCCCATTCG/TGCGCAGGTTGGTGTGAA | 63 | 87.9 ± 5.1 | NM_001007479.1 |
| Beta actin (β-actin) | GTCCACCGCAAATGCTTCTAA/TGCGCATTTATGGGTTTTGTT | 78 | 94.6 ± 6.4 | NM_205518.1 |
| Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) | GCCGTCCTCTCTGGCAAAG/TGTAAACCATGTAGTTCAGATCGATGA | 73 | 90.8 ± 5.4 | NM_204305.1 |
1
Primer efficiency is shown as mean ± SD.