Table 2.
Primer sequences used for quantitative real-time PCR assay.
| Gene name | Forward primer (5’–3’) | Reverse primer (5’–3’) | Length |
|---|---|---|---|
| β-actin | agtgtctttttgtatcttccgcc | ccacatactggcactttactccta | 147 bp |
| GPx-1 | tctacctggtaactttcgagcaa | cctttattgcagagcctcctt | 147 bp |
| GPx-4 | gccacctccatctacgacttc | ttggtgatgatgcagacgaag | 92 bp |
| SOD-1 | atgcagataggcacgtgg | actgccatcttaagcatttcag | 267 bp |
| TR-1 | tcaagaatgtcaccgcaagtt | cacgcagataacatccccaat | 129 bp |
| TR-3 | tgttttgatagccattggtcg | cataaggcacattggttcgttc | 128 bp |
Abbreviations: GPX-1, cellular glutathione peroxidase; GPX-4, phospholipid hydroperoxide glutathione peroxidase; SOD-1, superoxide dismutase 1; TR-1, thioredoxin reductase1; TR-3, thioredoxin reductase3.