Table 1.

Primers used in this study.

Target gene	Primers	Sequence (5′-3′)	Amplicon size (bp)	Annealing temperature (°C)	References
PBP5	PBP5F	CATGCGCAATTAATCGG	444	55	(Lleo et al., 1999)
	PBP5R	CATAGCCTGTCGCAAAAC
optrA	optrAF	AGGTGGTCAGCGAACTAA	1,395	53	(Wang et al., 2015)
	optrAR	ATCAACTGTTCCCATTCA
cfr	cfrF	TGAAGTATAAAGCAGGTTGGGAGTCA	746	48	(Kehrenberg and Schwarz, 2006)
	cfrR	ACCATATAATTGACCACAAGCAGC
fexA	fexAF	GTACTTGTAGGTGCAATTACGGCTGA	1,272	58	(Kehrenberg and Schwarz, 2006)
	fexAR	CGCATCTGAGTAGGACATAGCGTC
ermA	ermAF	TAACATCAGTACGGATATTG	200	54	(Di Cesare et al., 2013)
	ermAR	AGTCTACACTTGGCTTAGG
ermB	ermBF	CCGAACACTAGGGTTGCTC	139	54	(Di Cesare et al., 2013)
	ermBR	ATCTGGAACATCTGTGGTATG
mef	mefF	AGTATCATTAATCACTAGTGC	348	54	(Di Cesare et al., 2013)
	mefR	TTCTTCTGGTACTAAAAGTGG
tetL	tetlF	ATAAATTGTTTCGGGTCGGTAAT	1,077	52	(Aarestrup et al., 2000)
	tetlR	AACCAGCCAACTAATGACAATGAT
tetM	tetmF	GTTAAATAGTGTTCTTGGAG	657	53	(Aarestrup et al., 2000)
	tetmR	CTAAGATATGGCTCTAACAA
aac(6″)Ie-aph(2″)-la	aac6F	CAGAGCCTTGGGAAGATGAAG	348	55	(Vakulenko et al., 2003)
	aac6R	CCTCGTGTAATTCATGTTCTGGC
ant(6)-Ia	ant6IaF	ACTGGCTTAATCAATTTGGG	577	55	(Sepúlveda et al., 2007)
	ant6IaR	GCCTTTCCGCCACCTCACCG
asa	asa11	CACGCTATTACGAACTATGA	375	56	(Billström et al., 2008)
	asa12	TAAGAAAGAACATCACCACGA
ace	ace1	GGAATGACCGAGAACGATGGC	616	58	(Billström et al., 2008)
	ace2	GCTTGATGTTGGCCTGCTTCCG
cyt I	cyt I	ACTCGGGGATTGATAGGC	688	56	(Billström et al., 2008)
	cyt IIb	GCTGCTAAAGCTGCGCTT
efaA	efaA1	CGTGAGAAAGAAATGGAGGA	499	58	(Billström et al., 2008)
	efaA2	CTACTAACACGTCACGAATG
Esp	esp14 F	AGATTTCATCTTTGATTCTTG	510	56	(Billström et al., 2008)
	esp12 R	AATTGATTCTTTAGCATCTGG
Gel	gel11	TATGACAATGCTTTTTGGGAT	213	56	(Billström et al., 2008)
	gel12	AGATGCACCCGAAATAATATA
Hyl	hyln1	ACAGAAGAGCTGCAGGAAATG	276	56	(Billström et al., 2008)
	hyln2	GACTGACGTCCAAGTTTCCAA