Table 1.
Gene name | Primer sequence (5′-3′) | Product size | Accession No. |
---|---|---|---|
CD40 | F: GGCACCTTCTCCAATGTATCTTC | 96 | NM_204665 |
R: GTTCGTCCCTTTCACCTTCAC | |||
CD80 | F: CAGCAAGCCGAACATAGAAAGA | 270 | NM_001079739 |
R: AGCAAACTGGTGGACCTGAGA | |||
CD83 | F: GCTGACTTGCCTCGGGATT | 272 | XM_418929.5 |
R: TCACTCCGCTATCCGTCTCA | |||
CD197 | F: GACGACTATGACGCCAACAC | 211 | NM_001198752 |
R: CCAGGTTCAGCAAGTAGATGTC | |||
IL-6 | F: CTCCTCGCCAATCTGAAGTC | 99 | NM_204628 |
R: CCTCACGGTCTTCTCCATAAAC | |||
IL-1β | F: CGACATCAACCAGAAGTGCTT | 298 | NM_204524 |
R: GTCCAGGCGGTAGAAGATGA | |||
TNF-α | F: GGACAGCCTATGCCAACAAG | 81 | NM_204267 |
R: GCGGTCATAGAACAGCACTAC | |||
IL-10 | F: ACCAGTCATCAGCAGAGCAT | 222 | NM_001004414 |
R: CCTCCTCATCAGCAGGTACTC | |||
iNOS | F: TACTCTTGGCGTCATTACTC | 67 | NM_204961 |
R: GCATAGATCACAGTCACCTT | |||
TLR1 | F: CCGTTCAAGTGTTCGTGTGA | 116 | NM_001007488 |
R: CCGCTCAAGTCTTCTGGGTA | |||
TLR2 | F: TGTTCCTGTTCATCCTCATCCT | 168 | NM_204278 |
R: AGTTGGAGTCGTTCTCACTGT | |||
TLR4 | F: GAATGACACGGACACTCTT | 95 | NM_001030693 |
R: ACATAGGAACCTCTGACAAC | |||
TLR15 | F: CTTGTCGTTCTGGTGCTAA | 156 | NM_001037835 |
R: ATCGTGCTCGCTGTATGA | |||
MyD88 | F: GGATGGTGGTCGTCATTTCA | 225 | NM_001030962 |
R: GAGATTTTGCCAGTCTTGTCCA | |||
TRAF6 | F: ATATCCAGTTACCAAGTGCTCAG | 91 | XM_004941548 |
R: CAAGGCAGATGGCTGTCATAT | |||
NF-κB p65 | F: CTTCAATGTGCCAATGGAGGAG | 271 | NM_205129 |
R: CTCAGCCCAGAAACGAACCT | |||
JNK | F: GTAGTAGATCCAGACAAGAGAA | 71 | NM_205095 |
R: TCATACCAGACCGTAATATAGG | |||
β-actin | F: TATGTGCAAGGCCGGTTTC | 110 | NM_205518 |
R: TGTCTTTCTGGCCCATACCAA |
Abbreviations: CD, cluster of differentiation; F, forward; IL, interleukin; iNOS, inducible nitric oxide synthase; JNK, c-Jun N-terminal kinase; MyD88, myeloid differentiation primary response 88; NF-κB p65, nuclear factor kappa-B p65 subunit; R, reverse; TLR, toll like receptor; TNF-α, tumor necrosis factor alpha; TRAF6, TNF receptor associated factor 6.