Table 4.

Treatment by day interaction for gizzard and small intestine weights relative to BW (%) of broilers in ovo inoculated with different concentrations of the probiotic Enterococcus faecium from GalliPro Hatch (GH): MV (Marek's vaccine with not addition of probiotic), 10⁵ cfu GH (MV + 10⁵ cfu GH/50 μL), 10⁶ cfu GH (MV + 10⁶ cfu GH/50 μL), and 10⁷ cfu GH (MV + 10⁷ cfu GH/50 μL).¹

Day of grow-out	Gizzard				Duodenum				Jejunum
	MV alone	105 cfu GH	106 cfu GH	107 cfu GH	MV alone	105 cfu GH	106 cfu GH	107 cfu GH	MV alone	105 cfu GH	106 cfu GH	107 cfu GH
Day 0	5.793b	8.955a	8.839a	8.867a	0.955g	1.752a,b	1.845a	1.848a	1.269i	2.409b,c,d	2.333c,d,e	2.658b
Day 7	4.062c	4.189c	4.161c	4.113c	1.606b,c,d	1.522c,d,e	1.593b,c,d	1.449c,d,e	2.959a	2.621b	2.577b,c	2.554b,c
Day 14	3.028d	3.277d	3.063d	2.985d	1.343e,f	1.401d,e	1.643a,b,c	1.350e	2.207d,e,f	2.106e,f,g	2.440b,c,d	2.004f,g,h
Day 21	2.328e	2.231e	2.155e	2.007e	1.094g	0.985g	1.123f,g	1.108g	1.813h	1.838g,h	2.067e,f,g,h	1.934f,g,h
P value	0.0001				0.0001				0.0001
SEM	0.1593				0.0803				0.0984

Day of grow-out	Ileum				Ceca
	MV Alone	105 cfu GH	106 cfu GH	107 cfu GH	MV Alone	105 cfu GH	106 cfu GH	107 cfu GH
Day 0	0.896f	2.015b,c	2.140b	2.083b	0.606g,h	0.78b,c,d,e,f	0.910a,b	0.850a,b,c,d
Day 7	2.476a	2.214b	1.993b,c	1.991b,c	0.974a	0.790b,c,d,e,f	0.893a,b,c	0.796b,c,d,e,f
Day 14	1.648d,e	1.616d,e	1.835c,d	1.511e	0.685e,f,g,h	0.829a,b,c,d,e	0.737c,d,e,f,g	0.644f,g,h
Day 21	1.485e	1.546e	1.452e	1.526e	0.717d,e,f,g,h	0.688d,e,f,g,h	0.576g,h	0.564h
P value	0.0001				0.0001
SEM	0.0842				0.0583

For each tissue, means in a row and column not sharing a common superscript are different (P ≤ 0.05).

¹Means are calculated from 10 replicate values using one randomly chosen bird per pen and each pen as the experimental unit (10 pens/treatment, 16 birds/pen; 160 total birds/treatment).