Table 2.

Primers used for RT-qPCR amplification of chicken cytokines in this study.

Primer	F/R	Nucleotide sequence (5′- 3′)	Accession No.
GAPDH	F	CAACACAGTGCTGTCTGGTGGTA	NM_204305
	R	ATCGTACTCCTGCTTGCTGATCC
IL-1β	F	5′ CCTGTCTCTGTCCCTACCCCCTA 3′	HQ739080
	R	5′ GTCAACGGGTGTGCTGCAGGAAC 3′
IL-6	F	5′ GCATTCACTGAGTTTCCACCATT 3′	AJ309540
	R	5′ GTAGCACTGAGGGACATGGTAAG 3′
IL-10	F	5′ GATTCAGATAGAAGTTCTGTGCC 3′	AJ621254.1
	R	5′ GGTAACTTCTCTAAACACAGCAG 3′

Abbreviations: IL-1β, interleukin-1β; IL-6, interleukin-6; IL-10, interleukin-10.