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. 2020 Aug 26;99(11):6173–6187. doi: 10.1016/j.psj.2020.08.008

Table 1.

Nucleotide sequences of primers used for qRT-PCR amplification.

Gene Sequence (5′-3′) Product length (bp) Annealing temperature (°C) Accession ID
DIAPH2 F:AACAGCTACGAACACAGGCT 207 59.7 XM_027465243.1
R:TAAGCGGCAGCGAAGGTAG
KMT2E F:AGTGAAACGTGCAGGGTCAT 170 59.9 XM_027463760.1
R:CAGCCGCTCATCCAAAAAGG
KSR1 F:ACTGAGTCAGGTGGGGAGTT 217 59.5 XP_015736661.1
R:AGGTCCATGACTGGAGGAGA
ESR2 F:CAGTGCTACCTGTGACCAGA 168 60.0 XM_021274553.2
R:TGCAGCCTTCACATGACCAG
ASCF2 F:TATGGTGGACTCCAAGCTGC 215 59.5 XP_012951750.1
R:TTTCGCCAATGATGTTGCCA
A2M F:ACAGTTAGCCTGGAAGAGCG 176 59.5 XM_027449874.1
R:TCCTGAATAGCAATCAAGGGGT
BMPR1B F:TTAGAGGGCTCGGACTTCCA 128 59.9 XM_013104107.3
R:CGATTTTTCAGCGGAGGCAG
ACVR1 F:GTCAGGCTTGCTGGCCC 162 60.3 XM_005027629.4
R:AACGATCTCCGTTCCCACAG
β-actin F:CGCAAATGCTTCTAAACC 167 55.9 NM_001310421.1
R:AGACTGCTGCTGATACCTT