Table 2.
Primer pairs used for quantitative reverse-transcriptase polymerase chain reaction analysis.
| Gene1 | Gene bank identification | Primer sequence, sense/antisense | Product size (bp)2 |
|---|---|---|---|
| GAPDH | NM_204305.1 | GCTAAGGCTGTGGGGAAAGT/ TCAGCAGCAGCCTTCACTAC |
161 |
| Cla-1 | NM_001013611.2 | TGGAGGATGACCAGGTGAAGA/ CGAGCCACTCTGTTGCCATA |
115 |
| Cla-2 | NM_001277622.1 | CCTGCTCACCCTCATTGGAG/ GCTGAACTCACTCTTGGGCT |
145 |
| ZO-1 | XM_015278981.2 | CAACTGGTGTGGGTTTCTGAA/ TCACTACCAGGAGCTGAGAGGTAA |
101 |
| ZO-2 | XM_025144669.1 | ATCCAAGAAGGCACCTCAGC/ CATCCTCCCGAACAATGC |
100 |
| Ocln | XM_026041453.1 | ACGGCAGCACCTACCTCAA/ GGCGAAGAAGCAGATGAG |
122 |
| iNOS | NM_204961.1 | CAGCTGATTGGGTGTGGAT/ TTTCTTTGGCCTACGGGT |
158 |
1
GAPDH, glyceraldehyde-3-phosphate dehydrogenase; Cla-1, Claudin-1; Cla-2, Claudin-2; ZO-1, Zonula Occludens-1; ZO-2, Zonula Occludens-2; Ocln, Occludin; iNOS, nitric oxide synthase.
2
bp, Base pairs.