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. 2020 Aug 26;99(11):5844–5857. doi: 10.1016/j.psj.2020.08.017

Table 4.

Fluorescein isothiocyanate dextran concentration (FITC-d, μg/mL) at 5 d postinoculation (dpi), and claudin-1 (Cla-1), claudin-2 (Cla-2), zonula occludens-1 (ZO-1), zonula occludens-2 (ZO-2), and occludin (Ocln) gene expression at 6 and 14 dpi according to dietary Arg levels (1.04, 1.14, 1.24, 1.34, and 1.44%) in broilers challenged (Cha) or unchallenged (Unch) with a mixed Eimeria spp. infection.

Challenge Arg 5 dpi
 6 dpi
 14 dpi
FITC-d Cla-1 Cla-2 ZO-1 ZO-2 Ocln Cla-1 Cla-2 ZO-1 ZO-2 Ocln
Cha 1.04 0.38 1.26 1.06 1.19 1.09 1.03 1.04 0.88 1.03 1.00 1.12
1.14 0.34 2.07 1.15 1.21 1.09 1.15 0.98 1.09 1.15 1.11 1.18
1.24 0.18 1.24 0.97 0.93 0.91 1.04 0.87 0.85 0.98 0.96 1.06
1.34 0.07 1.32 1.08 1.43 1.16 1.23 0.95 0.89 0.94 0.89 1.08
1.44 0.41 0.88 0.93 1.07 0.97 1.15 1.15 0.97 1.27 1.16 1.29
Unch 1.04 0.16 0.77 1.02 1.35 1.08 0.88 1.01 0.78 1.07 1.00 1.14
1.14 0.03 0.50 0.84 1.01 0.94 1.11 1.19 0.87 1.13 1.12 1.29
1.24 0.13 0.50 0.95 1.03 1.00 1.15 1.20 0.89 0.88 0.81 1.09
1.34 0.05 0.40 1.13 1.47 1.13 1.05 0.89 0.82 0.87 0.93 1.14
1.44 0.09 0.47 0.89 0.88 0.91 1.13 0.80 0.87 1.24 1.19 1.13
Challenge Cha 0.28a 1.35a 1.04 1.17 1.04 1.12 1.00 0.94 1.07 1.02 1.15
Unch 0.09b 0.53b 0.97 1.15 1.01 1.06 1.02 0.85 1.04 1.01 1.16
Arg 1.04 0.27a 1.01 1.04 1.27a,b 1.08 0.96 1.02 0.83 1.05a,b 1.00a,b 1.13
1.14 0.18a,b 1.28 0.99 1.11a,b 1.01 1.13 1.08 0.98 1.14a,b 1.12a,b 1.24
1.24 0.16a,b 0.87 0.96 0.98b 0.95 1.10 1.04 0.87 0.93b 0.88b 1.07
1.34 0.06b 0.86 1.11 1.45a 1.15 1.14 0.92 0.86 0.90b 0.91a,b 1.11
1.44 0.25a,b 0.67 0.91 0.97b 0.94 1.14 0.98 0.92 1.25a 1.17a 1.21



SEM Challenge 0.044 0.175 0.098 0.116 0.069 0.075 0.145 0.068 0.091 0.071 0.068
Arg 0.025 0.101 0.057 0.067 0.039 0.043 0.083 0.039 0.052 0.041 0.039
Interaction 0.018 0.071 0.040 0.047 0.028 0.031 0.059 0.028 0.037 0.029 0.028



P-value Challenge <0.001 <0.001 0.409 0.843 0.562 0.408 0.897 0.152 0.668 0.844 0.840
Arg 0.019 0.258 0.650 0.012 0.129 0.397 0.970 0.588 0.038 0.012 0.368
L1 0.269 0.079 0.636 0.463 0.461 0.122 0.636 0.806 0.550 0.524 0.896
Q1 0.008 0.480 0.757 0.922 0.931 0.345 0.913 0.784 0.040 0.033 0.438
Interaction 0.060 0.237 0.703 0.661 0.783 0.667 0.641 0.769 0.983 0.863 0.704
L × Unch2 0.586 0.420 0.945 0.326 0.615 0.207 0.343 0.681 0.863 0.540 0.588
L × Cha2 0.310 0.091 0.461 0.958 0.588 0.353 0.772 0.948 0.501 0.772 0.453
Q × Unch3 0.552 0.585 0.944 0.867 0.765 0.300 0.344 0.659 0.072 0.052 0.880
Q × Cha3 0.002 0.128 0.712 0.976 0.859 0.771 0.423 0.957 0.257 0.271 0.208

a,bMeans followed by superscript letters are different by Tukey's test (P < 0.05) within the column.

1

L, Linear effect; Q, Quadratic effect.

2

L at Unch—Linear effect of Arg on unchallenged birds; L at Cha–Linear effect of Arg on challenged birds.

3

Q at Unch—Quadratic effect of Arg on unchallenged birds; Q at Cha–Quadratic effect of Arg on challenged birds.