Table 1.
Ingredient and calculated nutrient composition of experimental diets, as-fed basis.
| Item | α-amylase, KNU/kg | ||
|---|---|---|---|
| Ingredients, g/kg | 0 | 80 | 160 |
| Corn | 555.6 | 535.6 | 515.6 |
| Soybean meal | 360.0 | 360.0 | 360.0 |
| Soybean oil | 5.5 | 5.5 | 5.5 |
| Monocalcium phosphate1 | 11.0 | 11.0 | 11.0 |
| Limestone2 | 13.0 | 13.0 | 13.0 |
| Salt | 3.0 | 3.0 | 3.0 |
| Vitamin-mineral premix3 | 3.0 | 3.0 | 3.0 |
| DL-Methionine | 2.0 | 2.0 | 2.0 |
| L-Lysine HCl | 1.9 | 1.9 | 1.9 |
| Amylase premix4 | 0.0 | 20.0 | 40.0 |
| Titanium dioxide premix5 | 25.0 | 25.0 | 25.0 |
| Phytase premix6 | 20.0 | 20.0 | 20.0 |
| Total | 1,000.0 | 1,000.0 | 1,000.0 |
| Calculated nutrients and energy | |||
| Crude protein, g/kg | 228.2 | 228.2 | 228.2 |
| ME, kcal/kg | 3,005.5 | 3,005.5 | 3,005.5 |
| Ca, g/kg | 7.8 | 7.8 | 7.8 |
| P, g/kg | 6.2 | 6.2 | 6.2 |
| Nonphytate P, g/kg | 3.6 | 3.6 | 3.6 |
| Ca:total P | 1.3 | 1.3 | 1.3 |
| Ca:nonphytate P | 2.2 | 2.2 | 2.2 |
| Starch, g/kg | 439.6 | 439.6 | 439.6 |
| Total amino acids, g/kg | |||
| Arg | 14.8 | 14.8 | 14.8 |
| His | 6.0 | 6.0 | 6.0 |
| Ile | 9.4 | 9.4 | 9.4 |
| Leu | 19.4 | 19.4 | 19.4 |
| Lys | 13.7 | 13.7 | 13.7 |
| Met | 5.5 | 5.5 | 5.5 |
| Cys | 3.7 | 3.7 | 3.7 |
| Phe | 10.7 | 10.7 | 10.7 |
| Tyr | 8.8 | 8.8 | 8.8 |
| Thr | 8.5 | 8.5 | 8.5 |
| Trp | 3.0 | 3.0 | 3.0 |
| Val | 10.4 | 10.4 | 10.4 |
| Met + Cys | 9.1 | 9.1 | 9.1 |
| Phe + Tyr | 19.5 | 19.5 | 19.5 |
| Analyzed composition | |||
| Amylase (KNU/kg)7 | LOQ | 61 | 134 |
16% Ca, 21% P.
38% Ca.
Supplied the following per kg diet: vitamin A, 5,484 IU; vitamin D3, 2,643 ICU; vitamin E, 11 IU; menadione sodium bisulfite, 4.38 mg; riboflavin, 5.49 mg; pantothenic acid, 11 mg; niacin, 44.1 mg; choline chloride, 771 mg; vitamin B12, 13.2 ug; biotin, 55.2 ug; thiamine mononitrate, 2.2 mg; folic acid, 990 ug; pyridoxine hydrochloride, 3.3 mg; I, 1.11 mg; Mn, 66.06 mg; Cu, 4.44 mg; Fe, 44.1 mg; Zn, 44.1 mg; Se, 300 ug.
Ronozyme HiStarch contained 600 KNU/g. 1 g of HiStarch added to 149 g of corn supplied 4 KNU/g of premix. 20 g premix delivered 80 KNU/kg of feed and 40 g premix delivered 160 KNU/kg of feed.
Prepared as 1 g titanium dioxide added to 4 g corn.
Ronozyme HiPhos contained 5,000 FYT/g. 1 g of HiPhos added to 99 g of ground corn, supplied 50 FYT/g of premix. 20 g delivered 1,000 FYT/kg of feed. 1,000 FYT/kg supplied 1.5 g P/kg and 1.7 g of Ca/kg.
LOQ = limit of quantification.