Figure 3.

Results (mean ± 95% CI) for physiological parameters. The figures show glycerol concentrations (2 and 8%) and poloxamer 188 (P188) concentrations (0, 0.1, 0.5, 1%). When an interaction was found between both factors (HOS test, viability, apoptosis, dead sperm, apoptotic ratio, active mitochondria, ROS production, and ATP content), the levels were compared within each level of the other. Latin lowercase letters indicate differences among P188 concentrations within each glycerol concentration, whereas Greek letters show differences between glycerol concentrations within each treatment (P < 0.05). Plots with no letters indicate neither a significant effect of the factors nor interaction. Abbreviations: HOS, hypo-osmotic swelling; ROS, reactive oxygen species.