Table 1.
Composition and nutrient level of the basal diet (g/kg, as-fed basis unless otherwise stated).
| Items1 | 0 to 21 d | 22 to 42 d |
|---|---|---|
| Ingredients | ||
| Corn | 570.10 | 615.20 |
| Soybean meal | 315.00 | 250.00 |
| Corn gluten meal | 34.00 | 46.00 |
| Soybean oil | 31.00 | 41.00 |
| Limestone | 12.00 | 17.00 |
| Dicalcium phosphate | 20.00 | 14.00 |
| L-Lysine | 3.40 | 3.00 |
| DL-Methionine | 1.50 | 0.80 |
| Sodium chloride | 3.00 | 3.00 |
| Premix2 | 10.00 | 10.00 |
| Calculated nutrient levels | ||
| Apparent metabolizable energy (MJ/kg) | 12.55 | 13.06 |
| Crude protein | 213.32 | 194.87 |
| Calcium | 9.99 | 9.80 |
| Available phosphorus | 4.58 | 4.04 |
| Lysine | 12.09 | 10.41 |
| Methionine | 5.01 | 4.15 |
| Methionine + cystine | 8.56 | 7.47 |
| Analyzed composition | ||
| Crude protein | 209.41 | 188.74 |
| Calcium | 9.59 | 9.44 |
| Total phosphorus | 6.91 | 6.19 |
| Methionine | 4.79 | 3.94 |
| Lysine | 11.61 | 10.62 |
The squalene was added on top of the basal diet; The liquid squalene was mixed thoroughly with soybean oil used in broiler feed formulation before feed preparation at a level of 0 (Control group), 250, 500, 1,000, and 2,000 mg/kg, respectively.
Premix provided per kilogram of diet: vitamin A (transretinyl acetate), 10,000 IU; vitamin D3 (cholecalciferol), 3,000 IU; vitamin E (all-rac-α-tocopherol), 30 IU; menadione, 1.3 mg; thiamin, 2.2 mg; riboflavin, 8 mg; nicotinamide, 40 mg; choline chloride, 600 mg; calcium pantothenate, 10 mg; pyridoxine·HCl, 4 mg; biotin, 0.04 mg; folic acid, 1 mg; vitamin B12 (cobalamin), 0.013 mg; Fe (from ferrous sulfate), 80 mg; Cu (from copper sulphate), 8.0 mg; Mn (from manganese sulphate), 110 mg; Zn (from zinc oxide), 60 mg; I (from calcium iodate), 1.1 mg; Se (from sodium selenite), 0.3 mg.