Table 9.

Effect of SID Leu, Val, and Ile content in low-protein diets on mRNA expression of mTOR, S6K1, and eEF2 genes of the pectoralis major muscle of broiler chickens at 21 D of age (Experiment 4)¹.

SID amino acids (%)			mTOR	S6K1	eEF2
Leu	Val	Ile
1.28	0.65	0.54	0.0032	0.099	0.175c
1.28	0.65	1.09	0.0035	0.110	0.230a,b,c
1.28	1.2	0.54	0.0045	0.114	0.280a,b
1.28	1.2	1.09	0.0029	0.095	0.187b,c
1.83	0.65	0.54	0.0044	0.110	0.238a,b,c
1.83	0.65	1.09	0.0044	0.130	0.285a,b
1.83	1.2	0.54	0.0047	0.105	0.206b,c
1.83	1.2	1.09	0.0050	0.172	0.322a
SEM			0.0007	0.016	0.032
SID Leu levels (%)
1.28			0.0035b	0.105b	0.218
1.83			0.0046a	0.129a	0.263
SEM			0.0003	0.008	0.022
SID Val levels (%)
0.65			0.0039	0.113	0.232
1.2			0.0043	0.122	0.249
SEM			0.0003	0.008	0.022
SID Ile levels (%)
0.54			0.0039	0.108	0.225
1.09			0.0043	0.127	0.256
SEM			0.0003	0.008	0.022
Effects			P-value
Leu			0.01	0.03	0.10
Val			0.37	0.42	0.46
Ile			0.52	0.09	0.18
Leu x Val			0.86	0.39	0.53
Leu x Ile			0.38	0.04	0.03
Val x Ile			0.39	0.70	0.38
Leu x Val x Ile			0.21	0.10	0.02

^a,b,cMeans in columns followed by different superscript letters are statistically different (P < 0.05).

Abbreviations: eEF2, eukaryotic translation elongation factor 2; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; mTOR, mechanistic target of rapamycin.

¹Data are means of 5 replicate cages per treatment (treatments = 8; n = 40). Samples were analyzed in duplicate.