Figure 2.

β-NIK-OE Mice at Young Age Display Increased β Cell Death and Glucose Intolerance

(A) Islet β cell apoptosis in male β-NIK-OE mice and control mice at 8 weeks of age was measured by TUNEL assays (β-NIK-OE: n = 5; control: n = 5). (B) Relative insulin-positive areas (β cell mass) of male β-NIK-OE mice and control littermates at 8 weeks of age (β-NIK-OE: n = 6; control: n = 5). The scale bars represent 100 μm. (C and D) Glucose tolerance test (GTT) (C) and insulin tolerance test (ITT) (D) were measured in male β-NIK-OE and control mice at 8 weeks of age (β-NIK-OE: n = 6–7; control: n = 8). ∗p < 0.05, ∗∗p < 0.01. Data represent the mean ± SEM.