Figure 6.

Inhibition of NIK Activity by Small Molecule B022 Prevents NIK- or H₂O₂-Induced β Cell Failure In Vitro

INS-1 832/13 cells were infected with NIK adenovirus in the presence of B022 at different doses (0, 1, and 10 μM) for 16 h. (A) Flag-NIK, NF-κB2, and Tubulin protein levels were measured by immunoblotting. The quantification of p52/p100 was present on the right (n = 3 for each group). (B) Cell viability was measured by MTT (n = 6 for each group). (C) INS-1 832/13 cells were treated with H₂O₂ (50 μM) in the presence of B022 at different doses (0, 1, and 10 μM) for 16 h. Cell viability was measured by MTT (n = 5 for each group). (D–F) The expression of genes related to insulin secretion (Insulin1/2, Pdx-1, and Neurod1) (D), inflammation (CXCL1, IL1, IL6, and TNFα) (E), and antigen presentation (RT1-Ba, RT1-Da, and RT1-M2) (F) was measured by qRT-PCR (n = 5–6 for each group). ∗p < 0.05, ∗∗p < 0.01. Data represent the mean ± SEM.