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. 2020 Jun 30;11(11):852–857. doi: 10.1007/s13238-020-00746-2

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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OGT accumulates at the outer layer of the PCM and its localization changes during the ciliogenesis process. (A) MTECs fixed on ALI day 5 were subjected to immunofluorescence microscopy with antibodies against OGT and acetylated α-tubulin or centrin. (B) U2-OS cells were serum-starved and subjected to 3D-SIM with antibodies against OGT or different centrosome markers and centrin. (C) U2-OS cells were subjected to 3D-SIM with antibodies against OGT and γ-tubulin. (D) Quantification of the outer toroid diameters (nm) of the indicated centrosome proteins. (E) Summary of the toroid quantifications measured in D. n represents the number of centrosomes measured. Mann-Whitney test was performed for statistical analysis. (F) Model for the organization of the centrosome proteins. (G–I) MTECs were subjected to immunofluorescence microscopy for OGT and acetylated α-tubulin and grouped at different stages (G). The intensity of OGT and centrin at the basal bodies grouped to the indicated stages was then quantified by Mann-Whitney test (H and I). Scale bars, 10 μm unless specified. ****P < 0.0001; ns, not significant. Error bars indicate SD