Figure 1.

Overexpression or activation of ERK5 promotes migration of human lung cancer A549 cells and mouse melanoma cells. (A and B) Expression of ERK5 and p-ERK5 in lung cancer tissues and adjacent specimens. (A) Representative results of ERK5 and p-ERK5 staining micrographs of lung tissues (×20) analyzed by IHC. (B) The mRNA level of ERK5 was measured by RT-qPCR in human lung cancer sections. P1-L-P4-L indicated patient 1 to patient 4 with low grade. P1-H-P4-H indicated patient 1 to patient 4 with high grade. Data are represented as mean ± SD. (C) Wound healing assay was performed on ERK5-overexpression A549 cells (A549-ERK5). Relative scratch covered area was quantified by Image J from four areas. (D) Representative images of transwell-based cell invasion of ERK5-overexpression A549 cells. Matrigel was coated on the bottom of the well as the basement membrane matrix for invasion assay. The invasion cell numbers were quantified by Image J in 12 random fields from three independent experiments. (E) Relative cell viability of ERK5-overexpression A549 cells were measured by MTT assay. (F) Wound healing assay was performed on B16F1 cells transfected with MEK5D and ERK5 or control vector. Relative scratch covered area was quantified by Image J from four areas. (G) Wound healing assay was performed on B16F10 cells transfected with MEK5A and DN-ERK5 or control vector. Relative scratch covered area was quantified by Image J from four areas. Data are represented as mean ± SD, *P < 0.05 and **P < 0.01 compared with respective control or indicated treatment