Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Nov 6;11:5637. doi: 10.1038/s41467-020-19463-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 3 — IRF4^WT→ or IRF4^∆CD11c→ C57BL/6 bone marrow chimeras were immunized i.d. with Ms, Nb, Ca, or PBS as a control. LN cell suspensions were examined by flow cytometry on day 2 (a, b) or day 5 (c–k) after immunization. All conditions were compared within each experiment. a, b Cellular composition (a) and Ag median fluorescence intensity (MFI) (b) of LN Ag+ populations 2 days after immunization with CellTracker Orange-labeled Ms, Nb, or Ca. Graphs show mean ± SEM for groups of n = 9 (Ms and Nb IRF4^WT) or 8 (Ca IRF4^WT and all IRF4^∆CD11c) female chimeras from two independent experiments. Statistical significance was assessed using a two-sided Student’s t test for each cell population. NS: not significant, **p < 0.01, ****p < 0.0001. Exact values are shown for 0.05 > p > 0.01. c–k CD4+ T cell responses as assessed by flow cytometry. Intracellular cytokine staining was after in vitro restimulation, transcription factor (TF) expression was assessed without in vitro restimulation. c Numbers of CD4+ T cells per LN and (d) frequencies of CD44+ cells in the indicated CD4+ T cell populations. Bar graphs show mean values ± SEM, each symbol corresponds to one mouse. e Contour plots showing IFNγ staining of CD4+ T cells from Ms-immunized mice. Data are concatenated from 4 mice. f Frequencies of total cytokine+ and Tbet+ CD4+ T cells, or (g) IFNγ^hi (red gates in (e)) and Tbet^hi CD4+ T cells, in Ms-immunized mice. h, j Contour plots showing IL-4 and IL-17A staining of CD4+ T cells from Nb- or Ca-immunized mice, respectively. Data are concatenated from 4 mice. i, k Frequencies of cytokine+ and TF+ CD4+ T cells in Nb- or Ca-immunized mice. All graphs show mean ± SEM for groups of n = 6 (PBS IRF4^WT and PBS IRF4^∆CD11c) n = 7 (Ca IRF4^WT and Ms IRF4^∆CD11c) or n = 8 (Ms, Nb IRF4^WT and Nb, Ca IRF4^∆CD11c) female chimeras over two independent experiments; each symbol in (c, d) corresponds to one mouse. Statistical significance was assessed using Two-Way ANOVA with Sidak’s post-test. NS: not significant; ^##,**p < 0.01; ^###,***p < 0.001; ^####,****p < 0.0001. Hash symbols refer to comparisons between PBS and immunized chimeras of the same genotype. Asterisks refer to comparisons between similarly immunized IRF4^WT or IRF4^∆CD11c chimeras. Source data are provided as a Source Data file.