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. 2020 Sep 18;111(11):4232–4241. doi: 10.1111/cas.14640

FIGURE 2.

FIGURE 2

Temporal changes in the volume and intranodal blood vessel density of the tumor‐bearing subiliac lymph node (SiLN) with or without cis‐dichlorodiammineplatinum(II) (CDDP) treatment. The LN volume was measured on days 1, 3, and 6 (KM‐Luc/GFP; A, C) or days 3, 5, 7, 10, and 14 (FM3A‐Luc; B, D) using a contrast‐enhanced high‐frequency ultrasound imaging system. Dashed lines indicate SiLN borders. CDDP given with the lymphatic drug delivery system (LDDS) reduced the volume of SiLNs inoculated with KM‐Luc/GFP or FM3A‐Luc cells. Reconstruction of the intranodal blood vessels (green region) and calculation of blood vessel density were carried out using a high‐accuracy ultrasound contrast agent detection method on days 3 and 6 (KM‐Luc/GFP; E) or days 7 and 14 (FM3A‐Luc; F). The numbers of mice used in the study were: CDDP (n = 5), KM‐Luc/GFP + saline (n = 14), KM‐Luc/GFP + CDDP (n = 13), FM3A‐Luc + saline (n = 12), and FM3A‐Luc + CDDP (n = 11). Error bars indicate SEM. *P < .05, **P < .01