FIGURE 4.

Tumor explant supernatants induce lipid accumulation to generate cancer‐supporting M2 macrophages. A, Immunofluorescence images of lipid level in naïve peritoneal macrophages cultured with 10%, 30% MFC‐tumor explant supernatant (TES) for 24 h. B, Quantification of the intensity of BODIPY 493/503 by ImageJ. n = 5. C, D, Oil red O staining the lipid content in macrophage cultured with 30% MFC‐TES (Mag. 100×) (C) or with 30% MFC‐tumor‐conditioned medium (TCM) for 24 h (Mag. 600×) (D). E, F, Representative histogram showing lipid level (E) and quantification of the intensity (F) in naïve peritoneal macrophages cultured with indicated concentrations of MFC‐TES for 24 h. G, mRNA expression of the indicated typical M1 and M2 markers in macrophage cultured with control medium or 30% TCM for 48 h. The results are normalized to β‐actin. H, I, Representative histogram (H) and quantification (I) show that 30% of MFC‐TES‐treated macrophages have higher expression of CD206 and CD204 than naïve macrophages. n = 5; three experimental repeats. J, Immunofluorescence images of TCM‐treated macrophage. Scale bar, 20 µm. Red, F4/80; green, CD206; blue, DAPI