FIGURE 5.

Exogenous lipid mixture is sufficient to induce M2 polarization of macrophage. A, Immunofluorescence images of lipid level in naïve peritoneal macrophages cultured in the presence of vehicle or 2 µg/mL exo‐lipid mixtures for 24 h. B, Oil red O staining the lipid content in macrophage treated with 2 µg/mL exo‐lipid mixtures for 24 h (magnification 600×). C, Representative histogram showing lipid level (left panel) and quantification of lipid content (right panel) in naïve peritoneal macrophages treated with 2 µg/mL exo‐lipid mixtures or control medium for 24 h. D, mRNA expression of the indicated typical M1/M2 markers and typical cytokine genes in macrophage cultured for 48 h with the indicated condition. E, Representative histogram (left panel) and quantification (right panel) show that lipid mixture‐treated macrophages have higher expression of CD206 than control. n = 5; three experimental repeats