FIGURE 2.

PICSAR promotes cell proliferation, colony formation, and cell cycle progression and inhibits apoptosis in hepatocellular carcinoma cells in vitro. A, Huh7 and Hep3B cells that were transfected with corresponding vectors were subjected to quantitative RT‐PCR for PICSAR expression. Overexpression of PICSAR promoted cell proliferation (B), colony formation (C), and cell cycle progression (D) and inhibited apoptosis (E) in Huh7 cells. Downregulation of PICSAR inhibited cell proliferation (B), colony formation (C), and cell cycle progression (D) and promoted apoptosis (E) in Hep3B cells. F, Western blot analysis of cycle regulator Cyclin D1 and p21 and apoptosis‐related protein Bcl2/Bax expression in the presence and absence of PICSAR. n = three independent experiments. *P < .05, **P < .01