Fig. 1. Cellular compartment formation with length-varied FUS.

a Protein condensate formation of mCherry (mCh)-fused FUS constructs with varied lengths in HeLa cells (bottom) or length-varied FUS in a solution (middle). Schematic diagrams of length-varied FUS constructs (sequences in Supplementary Information) are shown (top). b Total mCh intensities of condensates (puncta sizes > 0.01 μm²) divided by whole cell mCh intensities. Data are presented as mean values with ±1 SD as error bars (n = 32 for 0.5 N, 0.5 C, 0.75, n = 31 for 1.0, 1.25, n = 33 for 1.5, 2.0 FUS; n: biologically independent cells examined over three independent experiments). c FRAP recovery images and profiles of scaffold proteins of mCh-1.5 FUS condensates. Data (point) are presented as mean values with ±1 SD as error bars (n = 33 cells from three independent experiments). Scale bars: 10 μm.