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. 2020 Nov 6;11:5642. doi: 10.1038/s41467-020-19476-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Schematic illustration of light-induced condensate formation by mCh-CRY2olig-IDR oligomerization and simultaneous IDR-Dronpa client turn-off (monomerization), followed by IDR-Dronpa recruitment and subsequent Dronpa tetramerization for (green) fluorescence turn-on inside condensates. b Fluorescence images of cells expressing mCh-CRY2olig-LAF (scaffold) with client DDX-Dronpa before and after 10 s 488 nm light activation and 4 min incubation. c Fluorescence images of cells expressing mCh-CRY2olig-LAF (scaffold) with free Dronpa (left) or cells with only DDX-Dronpa without scaffold (right) before and after 10 s 488 nm light activation and 4 min incubation. Scale bars: 10 μm. d Total GFP (tetramerized Dronpa)-to-mCh ratios for whole cells with various combinations of IDP scaffolds and clients. Data are presented as mean values with ±1 SD as error bars (n = 33 cells from three independent experiments).