Skip to main content
. 2020 Nov 6;11:5643. doi: 10.1038/s41467-020-19516-z

Fig. 4. Nup132 promotes HR-mediated DNA synthesis, downstream of Rad51 binding, in a post-anchoring manner.

Fig. 4

a Sensitivity of indicated strains to indicated genotoxic drugs. Ten-fold serial dilution of exponential cultures were dropped on appropriate plates. Bleo bleomycin; CPT camptothecin; HU hydroxyurea; MMS methyl methane sulfonate and UV: Ultra Violet-C. See supplementary Fig. 4 for the characterization of macromolecules transport and supplementary Fig. 5 for replication defect upon HU-fork stalling. b Frequency of RFB-induced Ura+ reversion in indicated strains and conditions. Each dot represents one sample from seven independent biological replicate for each strain. Bars indicate mean values ± SD. p value was calculated by two-sided t-test. c Binding of Rad51 to the RFB in indicated strains as described on Fig. 3e. Values are mean from n independent biological replicates ± SEM. d Co-localization event in S-phase cells in indicated conditions and strains. In all, 250 cells were analyzed for each condition and strain. p value was calculated by Fisher’s exact test for OFF and ON groups for each mutant and condition. Dots represent values obtained from two independent biological experiments. For each set of data, WT strain was analyzed alongside mutants. e Binding of Npp106-GFP to the RFB in indicated strains. Upstream and downstream distances from the RFB are presented in bp. Primers targeting ade6 gene were used as unrelated control locus. Values are mean of n independent biological repeats ± SD. p value was calculated using two-sided t-test.