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. 2020 Nov 6;11:5643. doi: 10.1038/s41467-020-19516-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a Representative RI analysis in indicated strains and conditions as described on Fig. 3. b Quantification of resected forks. Values are mean of four independent biological replicates ±SD. p value was calculated by two-sided t-test. c Binding of RPA (Ssb3-YFP) to the RFB in indicated strains. ChIP-qPCR results are presented as ON/OFF ratio for each mutant. Upstream and downstream distances from the RFB are presented in bp. Values are mean from four independent biological replicates ±SD. p value was calculated by two-sided t-test. Primers targeting ade6 gene were used as unrelated control locus. d SUMO-based regulation of relocation of remodeled forks to NPCs to promote RDR. See text for explanations.