Figure 3.

Repeat Dosing of AAV2.5T in Juvenile Ferrets

(A) Study design involving three groups of juvenile ferrets receiving 0, 1, or 2 doses of virus at 1 × 10¹³ DRP/kg via intra-tracheal administration. The ferrets receiving one dose were administered the reporter vector AAV2.5T-SP183-gLuc at 8 weeks old, whereas the ferrets receiving two doses were administered AAV2.5T-SP183-fCFTRΔR at 4 weeks old and AAV2.5T-SP183-gLuc at 8 weeks old. Plasma or BALF samples were collected at the indicated ages. (B) Gaussia luciferase activity in the plasma at the indicated time points after delivery of AAV2.5T-SP183-gLuc. (C) Gaussia luciferase activity in BALF at 14 days after delivery of AAV2.5T-SP183-gLuc. Results show the means ± SD for n = 9–10 animals per group. The statistical significance was analyzed by one-way ANOVA followed by Tukey’s multiple comparisons test on log₂-transformed data. ∗∗p < 0.01, ∗∗∗∗p < 0.0001.