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. 2020 Nov 6;10:19257. doi: 10.1038/s41598-020-76243-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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PBM effects on the skin microvasculature. CD31 marker epifluorescence images of the immunostaining for control and PBM-treated wounds at day 3 (a,b) and 7 post-surgery (c,d). In all groups and experimental times, the squared region is depicted in higher magnification (× 200) in the following image. PECAM-1 positive vessels (white arrows) are observed in the control (a,c) and in the PBM-treated samples (b,d) in both experimental times. Note the presence of an arteriole in a representative PBM sample (d). Also, note small capillaries in the photoactivated samples where the NG2 protein is observed colocalized with the CD31 marker (red arrowheads) (b,d). Scale bar = 100 µm.