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. 2020 Nov 6;10:19255. doi: 10.1038/s41598-020-76022-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of the bacterial decoy on variant identification by GATK HaplotypeCaller. Three loci are shown from internal sample A with genotype calls indicated by arrows before and after incorporation of the bacterial decoy. The left alignment shows SNVs called in heterozygous genotypes as a result of incorrectly aligned read pairs. The right alignment show those same sites after alignment to the decoy-supplemented genome.