Specific subject area |
Microbial biotechnology |
Type of data |
Genomic sequence, predicted genes and functional analysis of respective proteins |
How data was acquired |
De novo whole genome sequencing Instrument: Illumina MiSeq |
Data format |
Raw data: annotated draft genome assembly; Secondary data: table of orthologous gene clusters of Caldanaerobacter representatives; table of average nucleotide indentity between Caldanaerobacter genomes |
Parameters for data collection |
Thermophilic anaerobic pure culture cultivation. Extraction of genomic DNA from a pure culture, fragment library preparation, Illumina sequencing, de novo assembly and annotation procedures |
Description of data collection |
Extraction of genomic DNA was performed with ISOLATE II Genome DNA kit (Bioline, UK); fragment library was prepared with NEBNext Ultra kit; sequencing was performed with Illumina MiSeq™ system. The genome was assembled using Unicycler and annotated with NCBI PGAP web server |
Data source location |
The culture of strain 1523vc is deposited in extremophiles metabolism laboratory collection at Federal Research Center “Biotechnology” RAS (Moscow, Russian Federation) |
Data accessibility |
Raw data is publicly available at NCBI Genbank.The Biosample, Bioproject and assembly/WGS accession numbers are: SAMN14766777 (https://www.ncbi.nlm.nih.gov/biosample/SAMN14766777) PRJNA629090 (https://www.ncbi.nlm.nih.gov/bioproject/PRJNA629090) https://www.ncbi.nlm.nih.gov/bioproject/PRJNA511806) and JABEQB000000000 RZHM00000000 (https://www.ncbi.nlm.nih.gov/nuccore/JABEQB000000000), https://www.ncbi.nlm.nih.gov/nuccore/RZHM00000000), respectively. Secondary data is available as Supplementary Table 1 and 2. |
Related research article |
Kublanov IV, Perevalova AA, Slobodkina GB, et al. Biodiversity of thermophilic prokaryotes with hydrolytic activities in hot springs of Uzon Caldera, Kamchatka (Russia). Appl Environ Microbiol. 2009;75(1):286‐291. doi:10.1128/AEM.00607-08 |