Skip to main content
. 2020 Sep 24;33:106336. doi: 10.1016/j.dib.2020.106336
Subject Biology, Microbiology
Specific subject area Microbial biotechnology
Type of data Genomic sequence, predicted genes and functional analysis of respective proteins
How data was acquired De novo whole genome sequencing Instrument: Illumina MiSeq
Data format Raw data: annotated draft genome assembly; Secondary data: table of orthologous gene clusters of Caldanaerobacter representatives; table of average nucleotide indentity between Caldanaerobacter genomes
Parameters for data collection Thermophilic anaerobic pure culture cultivation. Extraction of genomic DNA from a pure culture, fragment library preparation, Illumina sequencing, de novo assembly and annotation procedures
Description of data collection Extraction of genomic DNA was performed with ISOLATE II Genome DNA kit (Bioline, UK); fragment library was prepared with NEBNext Ultra kit; sequencing was performed with Illumina MiSeq™ system. The genome was assembled using Unicycler and annotated with NCBI PGAP web server
Data source location The culture of strain 1523vc is deposited in extremophiles metabolism laboratory collection at Federal Research Center “Biotechnology” RAS (Moscow, Russian Federation)
Data accessibility Raw data is publicly available at NCBI Genbank.The Biosample, Bioproject and assembly/WGS accession numbers are: SAMN14766777 (https://www.ncbi.nlm.nih.gov/biosample/SAMN14766777) PRJNA629090 (https://www.ncbi.nlm.nih.gov/bioproject/PRJNA629090) https://www.ncbi.nlm.nih.gov/bioproject/PRJNA511806) and JABEQB000000000 RZHM00000000 (https://www.ncbi.nlm.nih.gov/nuccore/JABEQB000000000), https://www.ncbi.nlm.nih.gov/nuccore/RZHM00000000), respectively. Secondary data is available as Supplementary Table 1 and 2.
Related research article Kublanov IV, Perevalova AA, Slobodkina GB, et al. Biodiversity of thermophilic prokaryotes with hydrolytic activities in hot springs of Uzon Caldera, Kamchatka (Russia). Appl Environ Microbiol. 2009;75(1):286‐291. doi:10.1128/AEM.00607-08