Fig. 5.

Association of miRNA gene mutations with miRNA expression, patient survival, and cancer stage. (a) Heatmap shows miRNAs whose levels are significantly changed in samples mutated in the indicated genes or hotspots. Orange indicates that all miRNAs were downregulated by mutations in their genes. (b) Box plots show representative examples of miRNAs whose levels were changed in samples with mutations (mut) vs. samples without mutations (no-mut) in the corresponding gene. For Pan-Cancer analysis, miRNA levels were normalized to allow comparison between cancer types. (c) Heatmap shows the miRNA genes or hotspot mutations significantly associated with PFI (green - positively, orange - negatively). In the next columns, associations of the genes/hotspots with the other survival metrics (DFI, DSS, and OS) are also shown. (d) Example survival plots comparing mut and no-mut samples (shown in c). (e) Heatmap shows miRNA genes or hotspot mutations associated with cancer stages. Orange and green colours indicate associations of samples bearing mutations with higher and lower cancer stages, respectively. (f) Examples of associations (shown in e) of mutation with the distribution of cancer stages. To simplify the figure, we omitted the prefix hsa-miR in the gene IDs.