Fig. 1.

Recruitment flow diagram. The main phenotype derived from Arm 1 (visit 1) of the study, was stringently defined according to the criteria discussed under prescreening of records. Eighteen Arm 1 participants were excluded. We recalled as many negative IGRA participants as possible, since this was our phenotype of interest. Fifteen negative IGRA participants were excluded. Sixty-two of the part negative group had a negative second IGRA result. Four participants did not return. *Of the 58 participants who returned for a third visit, three had a positive third IGRA result (2 TST = 0mm, 1 TST = 15mm) and four did not have a third IGRA (TST = 7, 7, 10, 16mm). Fourty-eight participants have three negative IGRA results and a TST reading of 0 mm and were defined as HITTIN (indicated in blue on the flow diagram). °Eight participants tested IGRA negative on the first visit. They had a second positive IGRA result and retested IGRA negative the third time. Six of the eight had TST readings of 0 mm. These six participants likely had false positive IGRA results on the second IGRA test. The HIT group is indicated in green and was selected from 45 persons who tested IGRA positive for a second time and who had TST readings ≥5mm. The timeline on the left reflects the time between the first and the second IGRA of the HITTIN group (203±151 days) and then the time between second to the third IGRA (6±7 days). The timeline on the right reflects the time between the first and the second IGRA of the HIT groups (292±70 days). The TST were read 3 days later with only one read on day 4 and one on day 8. Both of these participants also self-reported persistant lack of TST response.