Figure 2.

DNMT3B Is Transcriptionally Regulated by CDK6 in BCR-ABL⁺ Cells

(A) Binding of CDK6 to the 5′ region of DNMT3B in BCR-ABL⁺ cells determined by ChIP-seq analysis (upper panel). Chromatin accessibility at the 5′ region of DNMT3B in BCR-ABL⁺ Cdk6^wt (green) and BCR-ABL⁺ Cdk6^−/− (red) cells determined by ATAC-seq analysis. See also Figure S1.

(B) Heatmap showing mRNA expression of DNMT1/3a/3b in BCR-ABL⁺ Cdk6^wt (green, N = 3) and BCR-ABL⁺ Cdk6^−/− (red, N = 3) cells analyzed by RNA-sequencing.

(C) Chromatin-bound DNMT1/3a/3b in BCR-ABL⁺ Cdk6^wt (green) and BCR-ABL⁺ Cdk6^−/− (red) cells analyzed by chromatome analysis. Mean values + SD are shown.

(D) ETS motifs found within the ATAC-seq peak at the 5′ UTR of DNMT3B.

(E) Binding of ETS1 to the 5′ region of DNMT3B in human K562 cells (left panel) and in murine spleen cells (right panel) determined by ChIP-seq analysis. See also Figure S1.