FIG 4.

Elevated osmotic strength impairs septal localization of DrpB. (A) Western blot with polyclonal anti-GFP serum. The arrow indicates DrpB-GFP. The identity of the strong band at 120 kDa is not known. (B) Septal localization of DrpB-GFP expressed from the native chromosomal locus in strain EC4680. (C) Septal localization of plasmid-expressed DrpB-GFP in a ΔdrpB background (EC4796). In the micrographs arrows point to septal localization and numbers refer to the fraction of cells scored positive for septal localization (mean ± SD from two experiments). Size bar = 10 μm. For this experiment, overnight cultures grown in LB were diluted into LB or LB0N; where indicated, LB0N was supplemented with NaCl, proline, or sucrose to a 200 mM final concentration.