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Inserting a single T into the wild-type covR promoter of strain A909 is sufficient to confer hyperhemolysis. (A) Schematic depiction of the covR upstream sequences of assayed strains. (B to D) Transcript levels of covR, the hemolysin gene cylE, and the CAMP factor gene cfb. *, P < 0.05 versus A909 (n = 4, one-way analysis of variance with Dunnett’s multiple-comparison test). (E) Hemolysis and pigment phenotype of GBS strains. (F) CAMP factor activity of GBS strains.
