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. 2020 Nov 2;12:11031–11040. doi: 10.2147/CMAR.S272983

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© 2020 Jiang et al.

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Circ-ATP5H directly interacts with miR-138-5p. (A) The binding sites between circ-ATP5H and miR-138-5p were displayed. (B and C) Luciferase activity was tested in Huh7-1.3 and HepG2.2.15 cells co-transfected with WT-circ-ATP5H or MUT-circ-ATP5H and miR-NC or miR-138-5p. (D and E) The levels of circ-ATP5H and miR-138-5p were measured in Huh7-1.3 and HepG2.2.15 cells transfected with si-NC, si-circ-ATP5H, pcDNA or circ-ATP5H. Huh7-1.3 and HepG2.2.15 cells were introduced with si-NC, si-circ-ATP5H, si-circ-ATP5H+anti-miR-NC or si-circ-ATP5H+anti-miR-138-5p, and miR-138-5p expression (F), HBV DNA copies (G), HBsAg and HBeAg levels (H and I) were determined using appropriate methods. ***P < 0.001, ****P < 0.0001.