Figure 7.

Apoptotic Efficacy of SMYD3i and PARPi Combined Treatment in a Panel of BC, CRC, PC, and OvCa Cell Lines

(A and B) Cell death analysis by annexin V staining. OVCAR-3 (A), BC (red), CRC (blue), PC (green), and OvCa (orange) cell lines (B) were pre-treated with a SMYD3i (100 μM BCI-121 or 10 μM EPZ031686) for 6 h, then treated with olaparib (10 μM) in the presence of the SMYD3i for a total of 72 h and analyzed by flow cytometry for annexin V staining. The indicated percentages of total apoptotic cells include early and late apoptotic and dead cells. Statistical analysis was performed using Student's t-test; ★p ≤ 0.05, all treatments compared with control; #p ≤ 0.05, combined treatments compared with the respective single treatments. Results are representative of at least three independent experiments. See also Figure S3.