Fig. 6.

Cellular accumulation of Galuminox either in serum and glucose deprived- or LPS-treated treated A549 cells either in presence or absence of Carvedilol (CAR),Dexrazoxane(DEX), and MitoTEMPO (MTT): Images were acquired using a 20× objective (all panels represent same magnification) in live human adenocarcinoma alveolar basal epithelial A549 cells. While A549 control cells were plated under normal conditions, both serum-and glucose-deprived (Starvation), and LPS treated cells were allowed to recover in presence of Carvedilol (CAR), Dexrazoxane (DEX), and MitoTEMPO (MTT) for 48 h, and treated with Galuminox (20 μM) for 60 min, cell uptake was evaluated via live-cell imaging, and quantified as described earlier [[50], [51], [52]]. Corrected Total Cell Fluorescence (CTCF) = Integrated Density–(Area of selected cell X Mean fluorescence of background readings); (mean ± SEM), where ***p < 0.001 and **p < 0.01 are determined by an unpaired student t-test.