Fig. 2.

Influence of the temperature, pH and choline on the activity of MSlys. a) The endolysin was incubated at different temperatures for 30 min, cooled on ice for about 20 min and then used to treat S. pneumoniae cells suspended in PBS. b) The endolysin was used to treat S. pneumoniae cells suspended in universal buffer at different pH. c) The endolysin was used to treat S. pneumoniae cells suspended in PBS containing different concentrations of choline. The reduction in the optical density reduction of S. pneumoniae R6st cells after treatment with MSlys (2 μM) for 2 h at 37 °C was monitored. Normalized data are shown as mean ± SD.