Table 1.
The spectrum of activity of MSlys.
| Species | Strain | Origin or Source | Growth conditions | Endolysin activity |
|---|---|---|---|---|
| Streptococcus pneumoniae | R6st | Félix d’Hérelle Reference Center for Bacterial Viruses | 37 °C, 5% CO2 | + |
| P046 | CIBER de Enfermedades Respiratorias, Madrid, Spain - Centro de Investigaciones Biológicas | + | ||
| MEF7_I1 (serotype 6A/B) | Middle ear fluid of children with otitis media, Hospital de Braga | + | ||
| MEF8_I2 | + | |||
| MEF12_I1 | + | |||
| MEF13_I1 | + | |||
| MEF14_I3 | + | |||
| MEF15_I3 (serotype 6A/B) | Middle ear fluid of children with otitis media, Trofa Saúde Hospital Braga Sul | + | ||
| MEF16_I1 | + | |||
| MEF18_I1 (serotype 19F) | + | |||
| MEF19_I1 | + | |||
| MEF26_I1 (serotype 6A/B) | + | |||
| MEF27_I1 | Middle ear fluid of children with otitis media, Trofa Saúde Hospital Braga Centro | + | ||
| MEF28_I1 | + | |||
| MEF29_I2 (serotype 6A/B) | + | |||
| MEF33_I2 (serotype 11A) | + | |||
| MEF35_I1 | + | |||
| C905005 (serotype 1) | Sputum, Hospital de Braga | + | ||
| I891301 (serotype 15B/C) | + | |||
| I895832 (serotype 1) | + | |||
| I903728 (serotype 19 F) | + | |||
| U944982 (serotype 4) | Blood culture, Hospital de Braga | + | ||
| Streptococcus mitis | I3124473 | Pus, Hospital de Braga | 37 °C, 5% CO2 | + |
| I310333 | Ocular, Hospital de Braga | + | ||
| U374030 | Pus, Hospital de Braga | + | ||
| Streptococcus agalactiae (Group B) | I302171 | Placenta, Hospital de Braga | 37 °C, 5% CO2 | – |
| I303139 | Perianal abscess, Hospital de Braga | – | ||
| Streptococcus anginosus (Anginosus group) | U365575 | Pus, Hospital de Braga | 37 °C, 5% CO2 | – |
| I298561 | Blood culture, Hospital de Braga | – | ||
| Streptococcus bovis (Group D) | U344929 | Urine, Hospital de Braga | 37 °C, 5% CO2 | – |
| U238779 | Pleural washout, Hospital de Braga | – | ||
| Streptococcus constellatus (Anginosus group) | I303868 | Periamydral abscess, Hospital de Braga | 37 °C, 5% CO2 | – |
| Streptococcus pyogenes (Group A) | C124992 | Ocular, Hospital de Braga | 37 °C, 5% CO2 | – |
| U164028 | Auricular, Hospital de Braga | – | ||
| Streptococcus salivarius | I299612 | Blood, Hospital de Braga | 37 °C, 5% CO2 | – |
| Streptococcus Group C | U269790 | Hemolysis, Hospital de Braga | 37 °C, 5% CO2 | – |
| Streptococcus Group G | U284971 | Hemolysis, Hospital de Braga | 37 °C, 5% CO2 | – |
| I196480 | Oropharynx, Hospital de Braga | – | ||
| Haemophilus influenzae | C894248 | Sputum, Hospital de Braga | 37 °C, 5% CO2 | – |
| Moraxella catarrhalis | U225012 | Ocular, Hospital de Braga | 37 °C, 5% CO2 | – |
| Staphylococcus aureus | ATCC 6358 | Human lesion, American Type Culture Collection | 37 °C | – |
| Pseudomonas aeruginosa | PAO1 (DSM 22644) | Infected wound, DSMZ – German Collection of Microorganisms and Cell Cultures GmbH | 37 °C | – |
| Enterococcus faecalis | I809 | Urine, Hospital de Braga | 37 °C | – |
The activity of MSlys was considered positive (+) if it resulted in a reduction of the optical density greater than 30 % after 2 h of incubation at 37 °C, using PBS as a negative control.