Table 2.

List of primers used to clone the endolysins and their main characteristics. The restriction sites of the enzymes are underlined.

Endolysin	Primer name	Primer sequence	Restriction enzyme	Tm (°C)	% GC	Product size (bp)
MSlys	MS_FW	GGTTTCATATGGGAGTAAATATTGATGAAGGCGTTGC	NdeI	59.2	41.4	906
	MS_RV	CCCAAGCTTCTACTTAGTAGTAATGAGCCCGTCAGG	HindIII	57.7	48.1
Pal	Pal_FW	GGTTTCATATGGGAGTCGATATTGAAAAAGGCGTTGC	NdeI	61.1	44.8	909
	Pal_RV	CCCAAGCTTTTAAACTTTAGCAGTAATGAGCCCGTCCGG	HindIII	61.7	46.7