Figure 1.

Fabrication of multi-phasic hydrogels. (a) Schematic of mold used for fabrication. (b) Schematic of fabrication method of multi-phasic hydrogels. Hydrogel precursor solution is layered with thermal crosslinking between layers before final UV crosslinking. (c), (f) Schematic of spatial organization of multi-phasic scaffolds. Note that each outlined box is a layer within the scaffold. Detailed explanation of fabrication of constructs corresponding to schematics (c) and (f) can be found in table 1. (d), (g) Representative fluorescent images of multi-phasic hydrogels containing fluorescently tagged BSA immediately following crosslinking. (e), (h) Representative graphs of linear intensity of fluorescent images from hydrogels in panels (d) and (g), respectively. Data are presented as representative images. Scale bar = 1 mm.