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. 2020 Nov 7;12:168. doi: 10.1186/s13148-020-00961-y

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — STXBP5-AS1 suppressed stemness of PC cells. a Sphere formation of PANC-1 and Mia PaCa-2 cells stably transfected with STXBP5-AS1 plasmid (pSin-STXBP5-AS1) or empty vector (pSin-vector). The total sphere numbers in each well were counted and images were taken at 40× magnification. b–d The expression levels of stem markers (Sox2, Bmi1, Lin28 and Nanog) in PANC-1 and Mia PaCa-2 cells stably transfected with STXBP5-AS1 plasmid (pSin-STXBP5-AS1) or empty vector (pSin-vector) were assessed by qRT-PCR and western blot. e Tumor incidence of PANC-1 cells stably transfected with STXBP5-AS1 plasmid (pSin-STXBP5-AS1) or empty vector (pSin-vector); cells were injected into the flank of mice with limiting dilutions as indicated. The number of tumors formed in each group was counted after 4 weeks. *P < 0.05; **P < 0.01