Table 2.
Primers Used for Amplification and Sequencing
| Primer name | Sequence |
|---|---|
| CO-PCR-F | 5′-TCAGAGGGAATTGAGCACCC-3′ |
| CO-PCR-R | 5′-GACACCCCACTCACGTCAT-3′ |
| CO-Seq-F | 5′-AAGAAGCTCTTCTGGAGGGCA-3′ |
| CO-Seq-R | 5′-AAGCGAGTTCCCAGTCAGGGA-3′ |
| CR-PCR-F | 5′-TGCATTCAAAGCTCCCACAT-3′ |
| CR-PCR-R | 5′-TCATTGAATGACTGCAACCC-3′ |
| CR-Seq-F1 | 5′-GGCATTTATAAGCATCTCTTGTTGGT-3′ |
| CR-Seq-R1 | 5′-CATATAGACCTGAGGGTAAGAAT-3′ |
| DI-PCR-F1 | 5′-AGATGTGCCCTACGTCAAGC-3′ |
| DI-PCR-R1 | 5′-TTAGGGGTCCAGCTCACTCA-3′ |
| DI-Seq-F1 | 5′-GGTACAGGACCCTTTTCTGGCT-3′ |
| DI-Seq-R1 | 5′-TGTCTGCCTCTTGACGCTCT-3′ |
| DO-PCR-F1 | 5′-CTGCAACCACATTCACCATCTG-3′ |
| DO-PCR-R1 | 5′-TCTGTGATCCTGAGTGGCCT-3′ |
| DO-Seq-F1 | 5′-AGTACCAAGGCTGTAGCAAACAG-3′ |
| DO-Seq-R1 | 5′-AATACCTTTTAGCAGCTGACAGTT-3′ |
| Duffy-PCR-F | 5′-CTGAGTGTAGTCCCAACCAGCCAA-3′ |
| Duffy-PCR-R | 5′-AGAGCTGCGAGTGCTACCTAGC-3′ |
| Duffy Seq-F1 | 5′-CAAGGCCAGTGACCCCCATA-3′ |
| Duffy Seq-R1 | 5′-GGCAAACAGCACGGGAAATGAG-3′ |
| Duffy Seq-F2 | 5′-GATGGCCTCCTCTGGGTATGTCCT-3′ |
| Duffy Seq-R2 | 5′-GCACCACAATGCTGAAGAGG-3′ |
| Kpab-PCR-F1 | 5′-GCCCCTCAACCCTTAATCCC-3′ |
| Kpab-PCR-R1 | 5′-TAGCAGCATCTCCTCCACCA-3′ |
| Kpab-Seq-F1 | 5′-TTGTGTCCATCTCCCAAGGC-3′ |
| Kpab-Seq-R1 | 5′-AGGGACTGAGAAGGGCTCAG-3′ |
| KEL578PCR-F | 5′-TTTAGTCCTCACTCCCATGCTTCC-3′ |
| KEL578PCR-R | 5′-TATCACACAGGTGTCCTCTCTTCC-3′ |
| KEL578SeqF | 5′-CCTAGAGGAATCGAAGGGCG-3′ |
| KEL578SeqR | 5′-CCAACTGTGTCTTCGCCAGT-3′ |
| JS-PCR-F2 | 5′-TTCCACCCTGGCTATCCCAG-3′ |
| JS-PCR-R1 | 5′-AGAAGGGCCATCAGGCTCTA-3′ |
| JS-Seq-F1 | 5′-TGCTGAGAGATTCTGGGGGT-3′ |
| JS-Seq-R1 | 5′-ATGGCCCTTGCTCACTGGTT-3′ |
| JK-PCR-F2 | 5′-CTATACCAGTGGGAGTTGGTCAGA-3′ |
| JK-PCR-R1 | 5′-GGGGAGCTTTGAGAAGGCAT-3′ |
| JK-Seq-F1 | 5′-ATTTTCCTGGGAGCCATCCT-3′ |
| JK-Seq-R1 | 5′-ACAGCAAGTGGGCTCAAGCC-3′ |
| KN-PCR-F1 | 5′-ACCATACTCTTCCTTCTCTCAGTCA-3′ |
| KN-PCR-R1 | 5′-CCCTCACACCCAGCAAAGTC-3′ |
| KN-Seq-F1 | 5′-GCAACAATAGAATAGAACATCTTTTCAC-3′ |
| KN-Seq-R1 | 5′-CACTCACCCCTGGAGCAGTGT-3′ |
| LU-PCR-F3 | 5′-AAAGAGGCAGAGCCAGGAGCTGCA-3′ |
| LU-PCR-R3 | 5′-TCACCACGCACACGTAGTCTCG-3′ |
| LU-Seq-F3 | 5′-GGGACACCCGGAGCTGAGAG-3′ |
| LU-Seq-R3 | 5′-TCACCACGCACACGTAGTCTCG-3′ |
| LW-PCR-F2 | 5′-CTTATCTCTAGAGCCGGCCCT-3′ |
| LW-PCR-R2 | 5′-AAGGGGGAGCCTCTGAGTGA-3′ |
| LW-Seq-F1 | 5′-TCTGTTCCCTCTGTCGCTGCT-3′ |
| LW-Seq-R1 | 5′-GCTCCCTATAGAGCGACTGTCA-3′ |
| IN-PCR-F1 | 5′-AGGTTCATGCCATTCTCCTG-3′ |
| IN-PCR-R1 | 5′-CCAGGAGAGCTCTGTGGAAG-3′ |
| IN-Seq-F1 | 5′-GGAGTCTGTCCTAAACTGAACTT-3′ |
| IN-Seq-R1 | 5′-GTCGGGTGCTGGTCTCTTACC-3′ |
| IN-Seq-F2 | 5′-TGTTAACCAGGCTGGTCTTGAG-3′ |
| IN-Seq-R2 | 5′-GGAAAGGAGCCTTCCAGTTC-3′ |
| OK-PCR-2–4F | 5′-GAGGTGTGGGGTTCATCAGT-3′ |
| OK-PCR-2–4R | 5′-TCCCCCTCGTTGATGTGTTC-3′ |
| OK-Seq-F | 5′-CCAGAGGTGTGGGGTTCATC-3′ |
| OK-Seq-R | 5′-TCAGGCCAACAACTTCTCCCGACA-3′ |
| SC-PCR-F1 | 5′-GGCGTCCCCAGAATAAGGAA-3′ |
| SC-PCR-R1 | 5′-GACACTTCCCTCTTGGGCAT-3′ |
| SC-Seq-F1 | 5′-TGGCAATCCTTTCCAGAGTCCTT-3′ |
| SC-Seq-R1 | 5′-ATTCCGGCATCAGAGATCTTCA-3′ |
| YT-PCR-F2 | 5′-CAGGAGAACGTGGCAGCCTT-3′ |
| YT-PCR-R | 5′-GGAGGACTTCTGGGACTTCTG-3′ |
| YT-Seq-F | 5′-ACCAGCGCAGGTCCTGGTGAA-3′ |
| YT-Seq-R | 5′-AATGGGCCTGGAGAAGCCCTCAT-3′ |
| CwCx-PCR-F4 | 5′-CCTCTTCTGAGCTTCAGTTTCCTTATTT-3′ |
| CwCx-PCR-R4 | 5′-GCTTCTAAAGGAAAGCTTACATTGTTGA-3′ |
| CwCx-Seq-F2 | 5′-CTCCATAGACAGGCCAGCACAG-3′ |
| CwCx-Seq-R2 | 5′-GAAGATGGGGGAATCTTTTCCTC-3′ |
| VVS-EePCR-F5 | 5′-CTGGGCAACAGAGCAAGAGTCC-3′ |
| VVS-EePCR-R5 | 5′-AGAAACGGGGTTCAAACTC-3′ |
| VVS-EeSeq-F | 5′-CCATCACAGAGCAGGTTCAGG-3′ |
| VVS-EeSeq-R | 5′-GGGGTGGGGAGGGGCATAAA-3′ |
| VVS-Fprimer 6 | 5′-AACAAACTCCCCATTGATGTGAGTA-3′ |
| VVS-Rprimer 6 | 5′-GCTCTGTGTTTGTGGGGTCACAG-3′ |
| VVS-Fprimer 3 | 5′-CCTTAGTGCCCATCCCCATTT-3′ |
| VVS-SeqR2 | 5′-ATCCAAGGTAGGGGCTGGACAT-3′ |
| RHCl09-PCRF3 | 5′-GTGCCACTTGACTTGGGACT-3′ |
| RHCl09-PCRR3 | 5′-GTGGACCCAATGCCTCTG-3′ |
| l09ins-Seq-3F | 5′-GGTACAATCATAGCTCATTGCTATAGC-3′ |
| l09ins-Seq-3R | 5′-CTATGATTGTACCACTGGGAAGTGAC-3′ |
| RHc307PCR-F3 | 5′-GCTTCCCCCTCCTCCTTCTCAC-3′ |
| RHc307PCR-R1 | 5′-TGAGAGGCCTTGAGAGGTCC-3′ |
| RHc307SeqF1-S | 5′-TGGGCTTCCTCACCTCAAAT-3′ |
| RHc307PCR-F4 | 5′-GTGCGAAAACAGTTGGTGATTATT-GATAAG-3′ |
| RHc307PCR-R4 | 5′-GGCAATATCCCAGATCTTCTGGAACC-3′ |
| RHc307SeqF4 | 5′-CAGTTGAGAACATTGAGGCTCA-3′ |
| RHc307SeqR4 | 5′-TTTCGGGGTCCATTCCCTCT-3′ |
| MN-PCR-F2 | 5′-GCTTTATCTGTAAACCTCTGCTATGC-3′ |
| MN-PCR-R3 | 5′-TGAGGTGACTGCGTGGACATAG-3′ |
| MN-Seq-F1 | 5′-GAGGGAATTTGTCTTTTGCAAT-3′ |
| MN-Seq-R2 | 5′-GGGTCTGAGCTGAACTCAGTTT-3′ |
| Ss-PCR-F3 | 5′-TAAGGCAACCATACTATCAATTGCTA-3′ |
| Ss-PCR-R3 | 5′-AGGCTTGGCCTCCCAAAATTATA-3′ |
| Ss-Seq-F2 | 5′-TGGCATCTCTGTGGAGTAATGGC-3′ |
| Ss-Seq-R2 | 5′-GTTAACAACATATGCTCTTCTG-TTTTAAG-3 |
| SSI-PCR-F5 | 5′-TGGGTCTGGAATCAGAAGCC-3′ |
| SSI-PCR-R5 | 5′-GACTTCTATGTGTCCAGTTGAAAAAG-3′ |
| SSI-Seq-F2 | 5′-CACATAATAGTATGTTAACTGTACTTTG-3′ |
| SSI-Seq-R4 | 5′-GAATTTTATGCAGTTCTGTTTCTCTTC-3′ |
| ABO-PCR-F6 | 5′-GGGCTGGGAATGATTTG-3′ |
| ABO-PCR-R6 | 5′-GGTGTCCCCCTCCTGCTATC-3′ |
| ABO-PCR-F1 | 5′-GGCAGAAGCTGAGTGGAGTT-3′ |
| ABO-O-SeqR2 | 5′-GCTCAGTAAGATGCTGC-3′ |
| ABO-PCR-F7 | 5′-CCCCGTCCGCCTGCCTTGCAG-3′ |
| ABO-PCR-R7 = R2 | 5′-GGGCCTAGGCTTCAGTTACTC-3′ |
| ABO-Bl-SeqF2 | 5′-CTACTATGTCTTCACCGAC-3′ |
| ABO-PCR-R1 | 5′-ACTCACAACAGGACGGACAA-3′ |
| DS4-S23 | 5′-GCCGACACTCACTGCTCTTAC-3′ |
| DS4-AS23 | 5′-TGAACCTGCTCTGTGAAGTGC-3′ |
| DS4-Seq23 | 5′-GGGAGATTTTTTCAGCCAG-3′ |
| RhD-Intron4-F24 | 5′-TAAGCACTTCACAGAGCAGG-3′ |
| Rh-I5R24 | 5′-TATGTGTGCTAGTCCTGTTAGAC-3′ |
| DS6-S23 | 5′-CAGGGTTGCCTTGTTCCCA-3′ |
| DS6-AS23 | 5′-CTTCAGCCAAAGCAGAGGAGG-3′ |