Table 1.
Evaluation of 24 protocols utilized for the production of PRF
| 100g | 200g | 400g | 700g | 1000g | 1200g | |
|---|---|---|---|---|---|---|
| Final volume (mL) | ||||||
| 3 min | 0.5 ± 0.29 | 1.25 ± 0.48 | 2.13 ± 0.31 | 2.25 ± 0.25 | 3.00 ± 0.41 | 3.75 ± 0.25 |
| 5 min | 1 ± 0.58 | 1.75 ± 0.25 | 2.75 ± 0.25 | 3.75 ± 0.48 | 4.75 ± 0.25 | 5.00 ± 0.41 |
| 8 min | 1.5 ± 0.65 | 3.25 ± 0.48 | 4.25 ± 0.48 | 5.00 ± 0.41 | 5.75 ± 0.25 | 5.75 ± 0.25 |
| 12 min | 2.5 ± 0.96 | 4.25 ± 0.48 | 5.00 ± 0.41 | 5.75 ± 0.25 | 5.75 ± 0.25 | 5.75 ± 0.25 |
| Total leukocytes (% total of 10 mL) | ||||||
| 3 min | 10.5 ± 0.71 | 18.42 ± 5.01 | 24.17 ± 3.08 | 24.33 ± 4.30 | 29.17 ± 5.11 | 35.67 ± 4.38 |
| 5 min | 14.17 ± 1.18 | 23.75 ± 2.67 | 31.92 ± 3.10 | 40.83 ± 8.75 | 53.58 ± 3.48 | 56.58 ± 3.33 |
| 8 min | 20 ± 7.07 | 40.79 ± 2.46 | 54.42 ± 5.63 | 62.83 ± 6.11 | 62.92 ± 2.75 | 62.17 ± 6.13 |
| 12 min | 36.75 ± 4.76 | 45.75 ± 2.47 | 55.75 ± 4.25 | 62.50 ± 4.98 | 62.25 ± 3.45 | 54.25 ± 4.57 |
| Concentration of leukocytes (% baseline) | ||||||
| 3 min | 95.83 ± 5.89 | 108.33 ± 6.31 | 128.47 ± 12.52 | 107.08 ± 12.52 | 97.57 ± 10.40 | 94.03 ± 6.59 |
| 5 min | 70.83 ± 5.89 | 141.67 ± 17.01 | 118.19 ± 12.90 | 107.64 ± 12.90 | 113.42 ± 7.59 | 114.76 ± 9.04 |
| 8 min | 79.17 ± 5.89 | 131.67 ± 14.06 | 128.63 ± 3.00 | 125.25 ± 9.09 | 110.56 ± 9.37 | 108.47 ± 10.36 |
| 12 min | 115.83 ± 4.12 | 96.71 ± 4.82 | 111.88 ± 3.37 | 109.17 ± 8.94 | 109.58 ± 10.83 | 95.69 ± 11.79 |
| Total platelets (% total of 10 mL) | ||||||
| 3 min | 10.35 ± 1.38 | 18.81 ± 3.04 | 43.63 ± 6.13 | 51.47 ± 5.24 | 68.75 ± 6.13 | 75.91 ± 4.91 |
| 5 min | 16.98 ± 1.92 | 42.49 ± 5.68 | 62.85 ± 5.61 | 82.76 ± 5.84 | 95.37 ± 1.61 | 94.98 ± 1.39 |
| 8 min | 29.17 ± 5.89 | 73.32 ± 7.82 | 89.03 ± 3.61 | 96.06 ± 2.33 | 94.92 ± 3.26 | 89.21 ± 1.64 |
| 12 min | 56.52 ± 0.92 | 84.88 ± 3.53 | 95.94 ± 1.71 | 94.09 ± 3.49 | 83.29 ± 6.89 | 81.69 ± 3.78 |
| Concentration of platelets (% baseline) | ||||||
| 3 min | 103.54 ± 13.85 | 148.37 ± 18.84 | 228.55 ± 17.33 | 229.56 ± 7.03 | 237.83 ± 19.39 | 202.37 ± 14.43 |
| 5 min | 87.40 ± 13.11 | 246.53 ± 15.47 | 230.32 ± 13.75 | 226.07 ± 17.03 | 202.67 ± 12.41 | 194.31 ± 17.79 |
| 8 min | 118.06 ± 9.82 | 232.06 ± 17.75 | 215.84 ± 19.59 | 196.29 ± 18.40 | 166.54 ± 12.08 | 156.45 ± 10.10 |
| 12 min | 176.82 ± 2.30 | 208.86 ± 17.64 | 195.88 ± 17.23 | 165.15 ± 12.57 | 146.76 ± 17.40 | 143.53 ± 12.30 |
Data includes final volume (mL), total leukocyte and platelet yields (% of the total from 10 mL) as well as concentration of leukocytes and platelets above baseline values (% increase) (data represents average ± standard error)