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. 2020 Nov 7;18:162. doi: 10.1186/s12951-020-00722-2

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Uptake of cancer cell-derived Reg-EVs and Br-EVs in human THP-1 monocytes. Breast cancer Reg EVs were derived from MDA-MB-231 cells and breast cancer Br-EVs from MDA-MB-231-BrM2-831 cells. Melanoma Reg-EVs were derived from A375 cells and melanoma Br-EVs from M12 melanoma brain metastases cells. Cell culture media were depleted of exogenous EVs and lipoproteins. a Flow cytometry and corresponding schematics of THP-1 cells incubated with fluorescently labeled breast cancer EVs. b Flow cytometry and corresponding schematics of THP-1 cells incubated with fluorescently labeled melanoma EVs for four hours. c Flow cytometry and corresponding schematic of HBMECs incubated with breast cancer EVs for four hours. LDL dose, 500 μg/10¹⁰ EVs. Data are presented as mean ± SD of three replicates. Statistical analysis was done using two-way ANOVA with post-hoc pairwise comparisons calculated with a Tukey’s test. ***p < 0.001; ****p < 0.0001