Table 3.
Integration sites of mobile genetic elements (MGE)
| MGE | Gene / contiga | PG21-homologueb | Gene product | Gene length (nt) |
Insertion site in gene (nt) | DR / IR c |
|---|---|---|---|---|---|---|
| FBG_ISMhom-1_1 | dBHBFJMJE_00070rc d | IGR e (dMHO_0640) | – | – | – | AAAATA |
| FBG_ISMhom-1_2 | dBHBFJMJE_00328 d | IGR e (dMHO_2560) | – | – | – | ATATTT |
| FBG_ISMhom-1_3 | dBHBFJMJE_00386 d |
IGR e (dMHO_3070) |
– | – | – | TAAATTATTTTGGGT |
| FBG_ISMhom-1_4 | BHBFJMJE_00532rc | MHO_4140rc | put. HAD hydrolase | 807 | 807 | AAATAAf |
| FBG_ISMhom-1_5 | BHBFJMJE_00625rc | MHO_5020rc | put. Sporulation transcription regulator WhiA | 828 | 828 | AAATAGGCf |
| SP10291_ISMhom-1 | dHPAMDCMO_00271 d | IGR e (dMHO_2210) | – | – | – | TGTGCTTTTT |
| 2539_MHoV1 | KLHMLDFE_00358 | MHO_3090 | hypothetical protein | 1134 | 808 | ATTTTTAT / ATTTTTTT |
| A136_MHoV1 | dMNIFKBBE_00264 d | IGR e (dMHO_2260rc) | – | – | – | TTTTTTTT / CTTTTTTT |
| SP2565_MHoV1 | dHHAOGLDO_00056 d | IGR e (dMHO_0530) | – | – | – | ATTTTTATA / ATTTTTCTA |
| 475_MHoV1 | dMOHCKDGE_00391 d | IGR e (dMHO_3470) | – | – | – | TTTTTT / CTTTTT |
| SS10_MHoV1 | MFOAEKDO_00548 | MHO_4930 | hypothetical protein | 903 | 867 |
CTTTTT (866–867) g |
| TO0613_MHoV1 | dKN71_002970 d | IGR e (dMHO_4930) | – | – | – | ATTTTTA |
| FBG_ICEHo-I-1rc | BHBFJMJE_00092 | MHO_0820 | hypothetical protein | 651 | 394 |
AAATAATA (387–394) g |
| FBG_ICEHo-I-2 | BHBFJMJE_00459 | MHO_3720 | P75 precursor | 1950 | 1950 + 1h | CAAATAAA/AATCTTT i (1323–1950) g |
| FBG_ICEHo-I-3 | BHBFJMJE_00651 | MHO_5300 | conserved hypothetical protein | 432 | 432 + 2h |
AAATAAAAf (427–432) g |
| FBG_ICEHo-I-4 vestige | BHBFJMJE_00287 | MHO_2250 | hypothetical protein | 663 | 44 | |
| FBG_ICEHo-I-5 vestigerc | dBHBFJMJE_00595 d |
IGR e (dMHO_4730) |
– | – | – | |
| SP10291_ICEHo-I | dHDENHCDK_00935 d | IGR e (dMHO_4550) | – | – | – | AAATTTTT |
| TO0613_ICEHo-I-1rc | dKN71_000220 d |
IGR e (dMHO_0170rc) |
– | – | – | TTTAAAAT |
| TO0613_ICEHo-I-2rc | KN71_001280 | MHO_2080 | conserved hypothetical lipoprotein | 1734 | 1407 |
TGGAAAT (1401–1407) g |
| PL5_ICEHo-I-1 |
JRX01A000001.1 |
MHO_0120 | type-III restriction enzyme | 2453 | 853 |
TTTTTAAA (846–853) g |
| PL5_ICEHo-I-2 | MHO_1960 | hypothetical protein | 369 | 4 |
AAATAAAG (4–12) g |
|
| SP3615_ICEHo-II | dKGPEAEHF_00485rc d |
IGR e (ddpnA) |
methylase DpnIIB | – | – | TAATATTA |
| SP10291_ICEHo-II | HPAMDCMO_00015 | MHO_0130 |
site-specific DNA methyl-transferase (DEAD/DEAH box helicase family protein) |
1200 | 1152 |
AAATCTTT (1145–1152) g |
aContigs only shown for strain PL5, due to assembly fragmentation; printed in italics; b MHO genes of PG21 according to acc.-no: FP236530.1; c DR = direct repeat; IR = inverted repeat; d dgene_X = insertion site downstream of gene X; rc = gene on reverse complementary strand; e IGR (= intergenic region) downstream of MHO-homologous gene X (dMHO_X); f downstream DR identical to 3′ end of the gene, protein encoding regions in bold; g DR region in the affected gene (nt x to nt y); h + 1 /+ 2 = direct repeat terminates 1 nt / 2 nt downstream of gene x resulting in downstream DR containing 3′ end of the gene; i no DR, but duplicated region of p75 (nt 1323–1950)