Table 5.
Sequence-based characteristics of ICEHo-II encoded proteins
| MICE-/ICEHo- | SP3615_ ICEHo-II | SP10291_ ICEHo-II | Homologues proteins | Species | Accession number | TMHMM v.2.0 | SignalP v. 5.0 | Motif | Reference of motif | DEPP S - T - Yc |
Protein length | Query cover | E-value | Identity |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CDS | KGPEAEHFa | HPAMDCMOa | BLASTp /[PSI-BLASTb] | (x/y) | (AA) | (%) | (%) | |||||||
| MICE_ CDS1 | _00500 | _00016 | ICEF-IA CDS1 | M. fermentans | AY168953.1 | – | – | rpoC2 | CHL00117 | 2/21–0/10–0/12 | 252 | 88.89 | 4.22E-03 | 36.60 |
| MhoM | _00499 | _00017 | hypothetical protein | M. hominis 4235 | WP_158532045.1 | – | – | – | – | 6/7–1/2–1/6 | 108 | 90.00 | 1.00E-58 | 87.76 |
| MhoM | _00498 | _00018 | hypothetical protein | M. hominis 4235 | WP_158532045.1 | – | – | – | – | 1/6–1/4–2/5 | 97 | 89.00 | 1.00E-21 | 48.31 |
| MhoN | _00497 | _00019 | hypothetical protein | M. hominis PL5 | WP_044329953.1 | – | – | – | – | 3/13–1/1–0/9 | 157 | 21.00 | 3.30E-01 | 61.76 |
| MhoO | _00496 | _00020 | hypothetical protein | M. primatum | WP_029513615.1 | – | – | – | – | 0/8–0/8–0/20 | 217 | 64.98 | 2.00E-14 | 37.59 |
| MhoP | _00495 | _00021 | variable lipoprotein | M. arginini | WP_129694644.1 | 1 | II | Lipoprot. 7 | pfam01540 | 0/14–0/13–1 / 14 | 272 | 45.96 | 3.86E-09 | 33.60 |
| MhoQ | _00494 | _00022 | hypothetical protein | I. bacterium | OIO22386.1 | – | – | AcrB | COG0841 | 0/4–0/3–0/2 | 93 | 59.00 | 7.40E-02 | 38.18 |
| MhoR | _00493 | _00023 | response regulator b | C. estertheticum | WP_152751962.1 | – | – | – | – | 1/10–0/2–0/2 | 102 | 87.00 | 9.90E-01 | 29.59 |
| MhoS | _00492 | _00024 | hypothetical lipoprotein | M. phocicerebrale | WP_116171775.1 | – | II | – | – | 5/27–1/21–2/22 | 415 | 99.00 | 1.00E-109 | 45.93 |
| BspA leu-rich repeat surface protein | M. bovis | WP_141790766.1 | – | – | 100.00 | 5.00E-101 | 13.17 | |||||||
| MhoT | _00491 | _00025 | hypothetical protein | M. phocicerebrale | WP_116171528.1 | 2 | – | – | – | 0/7–0/9–0/1 | 98 | 94.00 | 1.00E-19 | 72.04 |
| MhoU | _00490 | _00026 | hypothetical protein | M. phocicerebrale | WP_116171527.1 | 2 | – | – | – | 0/3–0/5–0/5 | 85 | 97.00 | 1.00E-14 | 42.86 |
| MICE_ CDS16 | _00489 | _00027 | hypothetical protein | M. phocicerebrale | WP_116171526.1 | 7 | – | smc_procA | TIGR02169 | 6/36–2/18–2/20 | 536 | 98.00 | 0.0 | 56.96 |
| ICEF_-A CDS16 b | M. fermentans | AAN85226.1 | 67.35 | 1.00E-34 | 14.20 | |||||||||
| MICE_ CDS17 | _00488 | _00028 | hypothetical protein | M. phocicerebrale | WP_116171521.1 | 3 / 2 | – | TraC_F-type | TIGR02746 | 0/58–5/49–1/35 | 885 | 99.00 | 0.0 | 65.34 |
| TraE/TraCb | Mycoplasma spp. | WP_013526664.1 | 99.00 | 0.0 | 37.02 | |||||||||
| MICE_ CDS19 | _00487 | _00029 | DNA polymerase III | M. phocicerebrale | WP_116171520.1 | 2 | I | polC | PRK00448 | 15/112–7/113–5/99 | 1821 | 99.00 | 0.0 | 44.38 |
| ICE-M CDS19b | M. mycoides | CBW53951.1 | 82.00 | 2.00E-123 | 26.29 | |||||||||
| MICE_ CDS22 | _00486 | _00030 | hypothetical protein | M. phocicerebrale | WP_116171517.1 | – | – | UPF0236 | pfam06782 | 0/21–0/12–0/24 | 387 | 100.00 | 8.00E-125 | 52.20 |
| ISLre2 transposase b | H. hemicellulosilytica | WP_125474456.1 | 99.00 | 9.00E-161 | 19.11 |
- = no detection; a number of the respective ICEHo_II ORFs in SP3615_(KGPEAEHF_00500 to _00468) and SP10291 (HPAMDCMO_00016 to _00030) according to Additional file 7; b homologous proteins identified by PSI-BLAST; c DEPP predicted phosphorylation sites (x) compared to total numbers of possible phosphorylation sites (y) of S serine, T threonine, Y tyrosine