Key resources table
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Histone H3 Antibody (mAb) | Active Motif | Cat No. 39064 |
| Bacterial and Virus Strains | ||
| DH5alpha (competent cells) | Macrolab | https://qb3.berkeley.edu/macrolab/ |
| MACH1 (competent cells) | Macrolab | https://qb3.berkeley.edu/macrolab/ |
| Biological Samples | ||
| None | ||
| Chemicals, Peptides, and Recombinant Proteins | ||
| Conconvalin A | Sigma-Aldrich | Cat No. C2272 |
| YNB w/o AA | BD Difco | Cat No. 233520 |
| Glucose | Sigma-Aldrich | Cat No. D9434 |
| Ammonium sulfate | Sigma-Aldrich | Cat No. 7783-20-2 |
| Sorbitol | Sigma-Aldrich | Cat No. S1876 |
| YNB w/o AA & ammonium sulfate | Life Sciences | Cat No. Y2030-02 |
| Calcium chloride | Fisher Scientific | Cat No. 50995817 |
| Complete supplement mixture | MP Biomedical | Cat No. MP114560122 |
| Dextrose | Sigma-Aldrich | Cat No. 50-99-7 |
| Yeast extract | Alfa Aesar | Cat No. 26769 |
| Peptone | BD Biosciences | Cat No. 211677 |
| Penicillin-Streptomycin (10,000 U/mL) | Thermo Fisher Scientific | Cat No. 15140122 |
| Formaldehyde | Thermo Fisher Scientific | Cat No. 28908 |
| 20X TBS, pH 7.4 | Teknova | Cat No. T1680 |
| Glycine | Fisher Scientific | Cat No. BP381 |
| Phenol-Chloroform | Thermo Fisher Scientific | Cat No. AM9720 |
| TE Buffer | Fisher Scientific | Cat No. BP24771 |
| Critical Commercial Assays | ||
| Quantseq 3’ mRNA-seq library prep kit FWD for Illumina | Lexogen | https://www.lexogen.com/quantseq-3mrna-sequencing/#quantseqdescription |
| Agilent RNA 6000 Pico Kit | Agilent Technologies | Cat No. 5067-1513 |
| Qubit dsDNA HS Assay Kit | Thermo Fisher Scientific | Cat No. Q32851 |
| Deposited Data | ||
| CLASP-Crz1 Data | This manuscript | http://dx.doi.org/10.17632/jxjnjmmj83.1 |
| Experimental Models: Cell Lines | ||
| None | ||
| Experimental Models: Organisms/Strains | ||
| Strain list, see Table S2 | This manuscript | N/A |
| Oligonucleotides | ||
| None | ||
| Recombinant DNA | ||
| Plasmid list, see Table S3 | This manuscript | N/A |
| Software and Algorithms | ||
| Matlab | Mathworks | https://www.mathworks.com/downloads/ |
| uManager | uManager | https://micro-manager.org/ |
| ilastik | ilastik | https://www.ilastik.org/ |
| FlowCytometryTools | Python | https://pypi.org/project/FlowCytometryTools/ |
| Python 2.7.12 | Python | https://docs.conda.io/projects/conda/en/latest/user-guide/install/index.html |
| LabView | National Instruments | https://www.ni.com/en-us/shop/labview/upgrade.html |
| STAR | Dobins 2013 | https://github.com/alexdobin/STAR |
| DESeq2 | Love 2014 | https://bioconductor.org/packages/release/bioc/html/DESeq2.html |
| SRA Toolkit | NCBI | https://www.ncbi.nlm.nih.gov/sra/docs/toolkitsoft/ |
| deepTools | deepTools | https://deeptools.readthedocs.io/en/develop/ |
| Arduino IDE | Arduino | https://arduino.en.softonic.com |
| CLASP-Crz1 Code | This manuscript | https://github.com/lindseyo/CLASP-Crz1 |
| Other | ||
| PhosphoGRID | PhosphoGRID - Mike Tyers | https://phosphogrid.org/ |
| CYCLoPs | University of Toronto - Andrews Lab | https://thecellvision.org/cyclops/ |