Table 1. Primer sequences used for PCR amplification of the enhancer activity detection and TFBS mutation analysis constructs.
| Constructs | Sequences (5′–3′) |
|---|---|
| ptnni1b-ECR183-E1b- GFP-T2KXIGQ | F: agagctcgagctgacagatagctgctgccggtagag R: agagagatcttgtctctttccctctgcagttgtctg |
| ptnni1b-ECR183-d1-E1b- GFP-T2KXIGQ | F: tcgagccaggagagaggagacgagcggttggagaggctggagatgctgcgagcattggtttcatcgctgtcataa R: gatcttatgacagcgatgaaaccaatgctcgcagcatctccagcctctccaaccgctc gtctcctctctcctggc |
| ptnni1b-ECR183-d2-E1b- GFP-T2KXIGQ | F: tcgagctgacagatagctgctgccggtagaggaaggagcatctccggcttggagacgaggactggactgaccgtggcgccaggagagaggaga R: gatctctcctctctcctggcgccacggtcagtccagtcctcgtctccaagccggagat gctccttcctctaccggcagcagctatctgtcagc |
| ptnni1b-ECR183-d3-E1b- GFP-T2KXIGQ | F: tcgagtgacagatagctgctgccggtagaggaaggagcatctccgga R: gatctccggagatgctccttcctctaccggcagcagctatctgtcac |
| ptnni1b-ECR183-h179-E1b- GFP-T2KXIGQ | F: agagctcgagccgagcggcagctcccacccacaga R: agagagatcttgccttctgctctccacccagctccc |
| ptnni1b-ECR183-h84-E1b- GFP-T2KXIGQ | F: tcgagccgagcggcagctcccacccacagagggcgcgtcaccagcctgcagccgcggactggactggccatagcgccatgacagagggga R: gatctcccctctgtcatggcgctatggccagtccagtccgcggctgcaggctggtgacgcgccctctgtgggtgggagctgccgctcggc |
| pGL3-E1b-tnni1b- ECR183 | F: agagggtaccctgacagatagctgctgccggtagag R: agagctcgagtgtctctttccctctgcagttgtctg |
| pGL3-E1b-tnni1b- ECR183-d2 | F: cctgacagatagctgctgccggtagaggaaggagcatctccggcttggagacgaggactggactgaccgtggcgccaggagagaggagc R: tcgagctcctctctcctggcgccacggtcagtccagtcctcgtctccaagccggagat gctccttcctctaccggcagcagctatctgtcagggtac |
| pGL3-E1b-tnni1b- ECR183-h179 | F: agagggtaccccgagcggcagctcccacccacaga R: agagctcgagtgccttctgctctccacccagctccc |
| pGL3-E1b-tnni1b- ECR183-h84 | F: cccgagcggcagctcccacccacagagggcgcgtcaccagcctgcagccgcggactggactggccatagcgccatgacagaggggc R: tcgagcccctctgtcatggcgctatggccagtccagtccgcggctgcaggctggtga cgcgccctctgtgggtgggagctgccgctcggggtac |
| pGL3-E1b-tnni1b- ECR183-d2-NKX2.5-mut | F: cctgacagatagctgctgccggtagaggaaggagcatcg(t)ccggcttggagacgaggactggactgaccgtggcgccaggagagaggagc R: tcgagctcctctctcctggcgccacggtcagtccagtcctcgtctccaagccggc(a) gatgctccttcctctaccggcagcagctatctgtcagggtac |
| pGL3-E1b-tnni1b- ECR183-d2-JUN-mut | F: cctgat(c)agatagctgctgccggtagaggaaggagcatctccggcttggagacgaggactggacg(t)gaccgtggcgccaggagagaggagc R: tcgagctcctctctcctggcgccacggtcc(a)gtccagtcctcgtctccaagccgga gatgctccttcctctaccggcagcagctatcta(g)tcagggtac |
Notes.
F:Forward primer sequence.
R:Reverse primer sequence.
Mutant bases are shown as bolded text, original bases are in parentheses.